Due to cause the deterioration of water quality and can produce toxic nitrite, the nitrate constituted of great threatens to human health and eco-systematic safety. Among most well-known biotechnology to remove nitrate, the integrated heterotrophic and autotrophic denitrification (IHAD) process is promising, especially for the organic-limited polluted water. In this work, the IHAD coupled manganese oxidation (IHAD-MnO) process was developed by using Pseudomonas sp. SZF15 (Gram negative strain, and rod-shaped morphology with 2.3 μm in length) in the glass serum bottles. It was found that limited organic content could accelerate nitrate removal rate, and manganese oxidation efficiency can reach up to 60.08%. To further explain carbon conversion characteristics of the process, pure heterotrophic condition assays were conducted, the results confirmed that inorganic carbon will be generated by organic carbon metabolism in heterotrophic condition, the maximum accumulation content of inorganic carbon was 142.21 mg/L (when the initial organic carbon level was 293 mg-C/L). Subsequently, since the consumption of organic carbon, biogenic inorganic carbon can be further utilized by microorganisms to support autotrophic denitrification (AuDN). Besides, X-ray photoelectron spectroscopy (XPS) was employed to analyze precipitation products produced from the process. The magnified Mn 2p spectra results showed that a typical characteristic peak of manganese dioxide was observed with the intense peak at 641.8 eV and a satellite peak at 653.7 eV, respectively. This showed that Mn(II) was oxidized to manganese dioxide by the process, which may be a functional material with adsorption properties. The process posed a highly efficient and cost effective solution with less carbon consumption and less greenhouse gas emission for sustainable water treatment technologies.

由于会导致水质恶化并产生有毒的亚硝酸盐，因此硝酸盐对人体健康和生态系统安全构成极大威胁。在最著名的用于去除硝酸盐的生物技术中，集成的异养和自养反硝化（IHAD）工艺很有希望，特别是对于有机物有限的污水。在这项工作中，使用假单胞菌开发了IHAD耦合锰氧化（IHAD-MnO）工艺sp。玻璃血清瓶中的SZF15（革兰氏阴性应变，长2.3μm的杆状形态）。结果表明，有限的有机物含量可以加快硝酸盐的去除速率，锰的氧化效率可以达到60.08％。为了进一步解释该过程的碳转化特性，进行了纯异养条件测定，结果证实了在异养条件下有机碳代谢将产生无机碳，无机碳的最大积累量为142.21 mg / L（初始时有机碳含量为293 mg-C / L）。随后，由于消耗了有机碳，微生物可以进一步利用生物无机碳来支持自养反硝化作用（AuDN）。除了，X射线光电子能谱（XPS）用于分析从该过程中产生的沉淀产物。Mn 2p光谱的放大结果表明，观察到典型的二氧化锰特征峰，强峰在641.8 eV，卫星峰在653.7 eV。这表明Mn（II）通过该过程被氧化成二氧化锰，这可能是具有吸附特性的功能材料。该过程为可持续水处理技术提出了一种高效且具有成本效益的解决方案，具有更低的碳消耗和更少的温室气体排放量。这表明Mn（II）通过该过程被氧化成二氧化锰，这可能是具有吸附特性的功能材料。该过程为可持续水处理技术提出了一种高效且具有成本效益的解决方案，具有更低的碳消耗和更少的温室气体排放量。这表明Mn（II）通过该过程被氧化成二氧化锰，这可能是具有吸附特性的功能材料。该过程为可持续水处理技术提出了一种高效且具有成本效益的解决方案，具有更低的碳消耗和更少的温室气体排放量。