High-throughput sequencing technologies brought a renewed interest for immune repertoires. Fish Ab and B cell repertoires are no exception, and their comprehensive analysis can both provide new insights into poorly understood immune mechanisms, and identify markers of protection after vaccination. However, the lack of genomic description and standardized nomenclature of IG genes hampers accurate annotation of Ig mRNA deep sequencing data. Complete genome sequences of Atlantic salmon and rainbow trout (Swanson line) recently allowed us to establish a comprehensive and coherent annotation of Salmonid IGH genes following IMGT standards. Here we analyzed the IGHV, D, and J genes from the newly released genome of a second rainbow trout line (Arlee). We confirmed the validity of salmonid IGHV subgroups, and extended the description of the rainbow trout IGH gene repertoire with novel sequences, while keeping nomenclature continuity. This work provides an important resource for annotation of high-throughput Ab repertoire sequencing data.

高通量测序技术为免疫库带来了新的兴趣。鱼类 Ab 和 B 细胞库也不例外，它们的综合分析既可以为了解甚少的免疫机制提供新的见解，也可以识别疫苗接种后的保护标记。然而，缺乏 IG 基因的基因组描述和标准化命名阻碍了 Ig mRNA 深度测序数据的准确注释。最近，大西洋鲑鱼和虹鳟鱼（斯旺森系）的完整基因组序列使我们能够按照 IMGT 标准对鲑鱼 IGH 基因进行全面和连贯的注释。在这里，我们分析了来自新发布的第二条虹鳟鱼系 (Arlee) 基因组的 IGHV、D 和 J 基因。我们确认了鲑鱼 IGHV 亚组的有效性，并用新的序列扩展了对虹鳟鱼 IGH 基因库的描述，同时保持了命名的连续性。这项工作为注释高通量抗体库测序数据提供了重要资源。