Pathogenic bacteria levels are significantly related with disease control, clinical diagnosis, and even environmental monitoring. It is becoming highly urgent to achieve ultrasensitive detection of pathogenic bacteria and efficient combat of bacterial infection. Toward this end, we have assembled a DNA Pom-Pom nanostructure (PP–N) based multifunctional platform for pathogenic bacteria determination and inactivation. In particular, one DNA oligonucleotide probe that serve as a trigger was specifically designed for the autonomous cross-opening of metastable DNA hairpin probes and long dsDNA structure formation, achieving a catalytic self-assembly of DNA nanostructure. Numerous DNA strands in this PP-N assembly provide sufficient interaction sites for functional domains and connector, showing high programmability, excellent biostability, as well as selective target recognition. With these properties, the fluorescence dyes modified PP-N platform showed excellent bacteria analysis with both excellent selectivity and ultrasensitive determination limit as low as 2.0 CFU/mL. Furthermore, the aptamer-functionalized and antibiotics loaded PP-N platform demonstrate excellent merits of high antibiotics-loading capacity and negligible cytotoxicity to targets. Therefore, this DNA PP-N assembly based multifunctional platform promise its great application in targeted sensing, combating bacterial infection, and potential clinic therapy.

病原细菌水平与疾病控制，临床诊断乃至环境监测密切相关。实现对致病菌的超灵敏检测和有效对抗细菌感染正变得迫在眉睫。为此，我们已经组装了基于DNA Pom-Pom纳米结构（PP–N）的多功能平台，用于病原细菌的测定和灭活。特别是，专门设计了一种用作触发器的DNA寡核苷酸探针，用于亚稳态DNA发夹探针的自主交叉开放和长dsDNA结构的形成，从而实现了DNA纳米结构的催化自组装。这种PP-N组件中的许多DNA链为功能域和连接器提供了足够的相互作用位点，显示出高度的可编程性，出色的生物稳定性，以及选择性的目标识别。凭借这些特性，荧光染料修饰的PP-N平台显示了出色的细菌分析，同时具有出色的选择性和低至2.0 CFU / mL的超灵敏测定限。此外，适体功能化和抗生素加载的PP-N平台具有高抗生素加载能力和对靶标的细胞毒性可忽略的优异优点。因此，这种基于DNA PP-N组装的多功能平台有望在靶向传感，对抗细菌感染和潜在的临床治疗中获得巨大的应用。适体功能化和抗生素装载的PP-N平台具有极高的装载量和对靶标的细胞毒性极低的优点。因此，这种基于DNA PP-N组装的多功能平台有望在靶向传感，对抗细菌感染和潜在的临床治疗中获得巨大的应用。适体功能化和抗生素装载的PP-N平台具有极高的装载量和对靶标的细胞毒性极低的优点。因此，这种基于DNA PP-N组装的多功能平台有望在靶向传感，对抗细菌感染和潜在的临床治疗中获得巨大的应用。