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Lignin Synthesis Related Genes with Potential Significance in the Response of Upland Cotton to Fusarium Wilt Identified by Transcriptome Profiling
Tropical Plant Biology ( IF 2 ) Pub Date : 2021-01-12 , DOI: 10.1007/s12042-020-09278-9
Jianan Hou , Fu’an Zhao , Xiaojie Yang , Wu Li , Deyi Xie , Zhongjie Tang , Shuping Lv , Lihong Nie , Yao Sun , Mengmeng Wang , Yajing Zhu , Xiaopei Bo , Qianwen Zhang , Yuanming Zhao , Weiping Fang

Fusarium wilt, caused by the fungus Fusarium oxysporum Schlecht. f. sp. vasinfectum (Fov) is a destructive soil-borne cotton disease. To profile the genes and pathways responding to Fov infection, we compared transcriptomic responses before and after F. oxysporum inoculation in a highly resistant cotton cultivar, Yumian21, and a highly susceptible cultivar, Jimian11. Although the overall gene expression pattern was downregulated in both cultivars, the global gene expression in the resistant cultivar was stronger than that in the susceptible cultivar. In addition, the expressed genes of two cultivars mostly differed in “cellular process,” “single-organism process,” “metabolic process,” and “response to stimulus” functional groups in the biological process Gene Ontology category: the upregulated differentially expressed genes (DEG) were largely enriched in the resistant cultivar, while the downregulated DEGs were largely enriched in the susceptible cultivar. Phenylpropanoid biosynthesis and phenylalanine metabolism are the key metabolic pathways in cotton in response to Fov. We found that lignin plays a potential role in cotton resistance to Fov. Two coding genes, caffeic acid 3-O-methyltransferase and peroxidase2, as well as the two transcription factors MYB46 and MYB86, are possibly involved in the accumulation and synthesis of lignin. Furthermore, the result showed that the quantification of lignin could be potentially used as a selection tool to identify Fusarium wilt resistant cotton.



中文翻译:

木质素合成相关基因在转录组分析鉴定中对陆地棉对枯萎病的响应具有潜在意义

枯萎病,由真菌尖孢镰刀菌引起F。sp。vasinfectum(Fov)是一种破坏性的土传棉花病。为了分析响应Fov感染的基因和途径,我们比较了F. oxysporum之前和之后的转录组反应在高抗性棉花品种Yumian21和高易感品种Jimian11中接种。尽管两个品种的总体基因表达模式均被下调,但抗性品种的整体基因表达要强于易感品种。此外,在生物学过程中,两个品种的表达基因在“细胞过程”,“单一生物过程”,“代谢过程”和“对刺激的反应”功能组中的差异最大:基因表达上调的差异表达基因(DEG)在抗性品种中大量富集,而下调的DEG在易感品种中大量富集。苯丙烷类生物合成和苯丙氨酸代谢是棉花响应Fov的关键代谢途径。我们发现木质素在棉花对Fov的抗性中起潜在作用。两个编码基因咖啡酸3-O-甲基转移酶过氧化物酶2以及两个转录因子MYB46MYB86可能参与木质素的积累和合成。此外,结果表明,木质素的定量可潜在地用作鉴定抗枯萎病棉花的选择工具。

更新日期:2021-01-12
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