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Genetic characterization and association mapping in near-isogenic lines of waxy maize using seed characteristics and SSR markers
Genes & Genomics ( IF 2.1 ) Pub Date : 2021-01-12 , DOI: 10.1007/s13258-020-01030-7
Hae Ri Kim 1 , Kyu Jin Sa 1 , Min Nam-Gung 2 , Ki Jin Park 2 , Si-Hwan Ryu 2 , Chang Yeun Mo 3 , Ju Kyong Lee 1
Affiliation  

Background

Association mapping has been advocated as the method of choice for identifying loci involved in the inheritance of complex traits in crop species. This method involves identifying markers with significant differences in allele frequency between individuals with a phenotype of interest and a set of unrelated control individuals.

Objective

The purpose of our study is not only to investigate the genetic diversity and relationships of the basic molecular markers of near-isogenic lines (NILs) of waxy maize, but it is also to identify molecular markers related to interesting seed characteristics including 4 seed quantity traits and 4 seed phenotypic traits using association analysis with population structure.

Methods

We performed association mapping of 200 SSR markers and 8 seed characteristics among 10 NILs of waxy maize and two parental lines (HW3, HW9) (recurrent parent) of “Mibaek 2” variety.

Results

In population structure and cluster analysis, the 10 NILs and two parental lines were divided into two groups. Seven inbred lines, including HW3, were assigned to Group I. Group II contained 5 inbred lines, including HW9. In addition, we found that 32 SSR markers associate with 8 seed characteristics in 10 NILs. In particular, five SSR markers (umc1986, umc1747, umc2275, phi078, umc1366) were together associated with more than one seed characteristics such as EL, 100 KW, SCC, R, L*, and V.

Conclusion

This study demonstrated the utility of SSR analysis for studying GD, population structure, and association mapping in 10 NILs and two parental lines (HW3, HW9) of “Mibaek 2” variety.



中文翻译:

使用种子特征和 SSR 标记的糯玉米近等基因系的遗传特征和关联作图

背景

关联作图已被提倡作为识别涉及作物物种复杂性状遗传的位点的首选方法。该方法涉及识别具有感兴趣表型的个体和一组不相关的对照个体之间等位基因频率显着差异的标记。

客观的

我们研究的目的不仅是研究糯玉米近等基因系(NILs)基本分子标记的遗传多样性和关系,而且还旨在鉴定与有趣的种子特征相关的分子标记,包括4个种子数量性状。和 4 个种子表型性状使用与种群结构的关联分析。

方法

我们对糯玉米的 10 个 NIL 和“Mibaek 2”品种的两个亲本系(HW3、HW9)(轮回亲本)之间的 200 个 SSR 标记和 8 个种子特征进行了关联作图。

结果

在种群结构和聚类分析中,10 个 NIL 和两个亲本被分为两组。包括 HW3 在内的 7 个自交系被分配到第一组。第二组包含 5 个自交系,包括 HW9。此外,我们发现 32 个 SSR 标记与 10 个 NIL 中的 8 个种子特征相关。特别是,五个 SSR 标记(umc1986、umc1747、umc2275、phi078、umc1366)一起与不止一种种子特征相关,例如 EL、100 KW、SCC、R、L* 和 V。

结论

本研究证明了 SSR 分析在研究“Mibaek 2”品种的 10 个 NIL 和两个亲本系(HW3、HW9)中的 GD、种群结构和关联作图方面的效用。

更新日期:2021-01-12
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