The present study was aimed to investigate brilliant green (BG) dye sorption onto soybean straw biochar (SSB) prepared at 800 °C and further understanding the sorption mechanism. Sorption kinetic models such as pseudo-first and pseudo-second order were executed for demonstrating sorption mechanism between the dye and biochar. Results of kinetics study were fitted well to pseudo-second-order kinetic model (R² 0.997) indicating that the reaction followed chemisorption mechanism. Furthermore, the effect of various parameters like sorbent dose, dye concentration, incubation time, pH and temperature on dye sorption was also studied. The maximum dye removal percentage and sorption capacity for SSB (800 °C) within 60 min were found to be 99.73% and 73.50 mg g^− 1, respectively, at pH 8 and 60 °C temperature, whereas adsorption isotherm studies showed a higher correlation coefficient values for Freundlich model (R² 0.990–0.996) followed by Langmuir model suggesting that sorption process was multilayer. The characterization of biomass and biochar was performed with the aid of analytical techniques like scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), Brunauer–Emmett–Teller (BET) theory, X-ray diffraction (XRD) and thermo-gravimetric analysis (TGA). FTIR analysis showed active groups on biochar surface. BET study revealed higher surface area of biochar (194.7 m²/g) than the biomass (12.84 m²/g). Besides, phyto- and cytogenotoxic studies revealed significant decrease in the toxicity of dye containing water after treating with SSB. Therefore, this study has proved the sorption potential of soybean straw biochar for BG dye and could be further considered as sustainable cost-effective strategy for treating the textile dye-contaminated wastewater.

本研究旨在研究亮绿 (BG) 染料在 800 °C 制备的大豆秸秆生物炭 (SSB) 上的吸附，并进一步了解吸附机制。执行吸附动力学模型，如伪一级和伪二级，以证明染料和生物炭之间的吸附机制。动力学研究结果与拟二级动力学模型（R ² 0.997）吻合，表明反应遵循化学吸附机制。此外，还研究了吸附剂剂量、染料浓度、孵育时间、pH 和温度等各种参数对染料吸附的影响。发现 SSB (800 °C) 在 60 分钟内的最大染料去除百分比和吸附容量为 99.73% 和 73.50 mg g ^{− 1}，分别在 pH 8 和 60 °C 温度下，而吸附等温线研究显示 Freundlich 模型（R ² 0.990–0.996）的相关系数值更高，Langmuir 模型表明吸附过程是多层的。借助扫描电子显微镜 (SEM)、傅里叶变换红外光谱 (FTIR)、Brunauer-Emmett-Teller (BET) 理论、X 射线衍射 (XRD) 和热分析技术等分析技术对生物质和生物炭进行表征。 -重量分析（TGA）。FTIR 分析显示生物炭表面存在活性基团。BET 研究表明，生物炭的表面积 (194.7 m ² /g) 比生物质 (12.84 m ² /g)/G）。此外，植物和细胞遗传毒性研究表明，用 SSB 处理后，含染料的水的毒性显着降低。因此，本研究证明了大豆秸秆生物炭对 BG 染料的吸附潜力，可进一步被视为处理纺织染料污染废水的可持续成本效益策略。