Using lignocellulosic raw materials as substrate for biotechnological applications has been a focus of research during the last two decades. They contain sugars, which can be used in industrial fermentation processes, in from of polysaccharides (cellulose, hemicellulose). Wheat straw, one representative of lignocellulosic materials, is sustainably and abundantly available, especially in Europe and North America. However, wheat straw, just like any other lignocellulosic material, needs to be pretreated in one way or the other in order to generate sufficient quantities of monosaccharides. One widely used pretreatment for lignocellulosic material is steam explosion combined with enzymatic hydrolysis. In this study, the effects of steam exploding wheat straw in combination with water are presented. By impregnation with water, saccharide yields from subsequent enzymatic hydrolysis increased from 18.8 to 22.6 g L⁻¹ for glucose and 13.8 to 16.4 g L⁻¹ for xylose, respectively. Moreover, the basic steam explosion parameters residence time and temperature were optimized in ranges from 5 to 20 min and 180–200 °C. This further optimization increased the maximum saccharide yield to 41.2 g L⁻¹ for glucose (200 °C, 15 min) and 18.9 g L⁻¹ for xylose (190 °C, 10 min). Finally, the growth of the intensively investigated biotechnological production host Yarrowia lipolytica on hydrolysates derived from different steam explosion parameters was evaluated. Y. lipolytica grew well in media containing up to 90% wheat straw hydrolysate as sole carbon source, demonstrating the potential as substrate for biotechnological processes.

在过去的二十年中，使用木质纤维素原料作为生物技术应用的基质一直是研究的重点。它们包含糖（可用于工业发酵过程）中的多糖（纤维素，半纤维素）中的糖。麦秸是木质纤维素材料的代表之一，可以持续且大量地获得，特别是在欧洲和北美。但是，小麦秸秆，就像任何其他木质纤维素材料一样，需要以一种或另一种方式进行预处理，以产生足够量的单糖。一种广泛用于木质纤维素材料的预处理方法是蒸汽爆破与酶促水解相结合。在这项研究中，提出了蒸汽使麦秸与水爆炸的效果。通过浸水，^-1葡萄糖和13.8至16.4克L- ^-1分别为木糖。此外，基本蒸汽爆炸参数的停留时间和温度在5至20分钟和180-200°C的范围内进行了优化。进一步的优化将最大糖产量（葡萄糖（200°C，15分钟）提高到41.2 g L ^-1和木糖（190°C，10分钟）达到18.9 g L ^-1。最后，评估了深入研究的生物技术生产宿主解脂耶氏酵母在源自不同蒸汽爆炸参数的水解产物上的生长。解脂耶氏酵母 在含有高达90％小麦秸秆水解产物作为唯一碳源的培养基中生长良好，证明了其作为生物技术工艺的底物的潜力。