The control region of mitochondrial genome is the main non-coding region. There is no details about Geospiza magnirostris’s control region, who is the only species of Geospiza in Darwin finches whose mitochondrial genome has been sequenced. In this study, the control region mitochondrial genome of G. magnirostris was analyzed. Compare research for 15 Passerine species, belonging to 4 families (Darwin ground finch, Thraupidae, Icteridae, Emberizidae), was also been done. The result showed the length was 1202 bp and it could been divided into three parts, domainI, domainII and domainIII. Genetic distances showed average divergence of different family ranged from 0.36% (Darwin ground finch) to 9.20% (Emberizidae), indicated that the Darwin ground finch may be a very conservative single-source species. Some specific sequence fragments such as conservative sequence box, boxes C, D, F and E, HSP/LSP, CSB-1, TAS-1 were also found in the control region of G. magnirostris, while B-box, CSB-2 and CSB-3 were not found in it. The F-box was detected in all selected 15 species and was 29 bp in length with no variant sites. In the E-box, 20 of the 21 nucleotide positions were identical as conserved among the selected species. There are two variant sites in the D-box, and five variant sites in the C-box. Basing the phylogenetic tree, it can be clearly seen that the relationship of G. magnirostris and family Thraupidae is the closest in all selected family. Through this study, the structure characteristics of G. magnirostris control region were explored and understood. This study provided more basic information for in-depth research of G. magnirostris. It’s also reminded us that we should pay more attention to explore the characteristics of the control region of Darwin’s finches.

线粒体基因组的控制区是主要的非编码区。目前尚无关于大孢Geospiza的控制区的详细信息。大孢Geospiza magnirostris的控制区是达尔文雀科中唯一已对线粒体基因组进行测序的大孢藻。在这项研究中，控制区域的线粒体基因组的G.巨嘴龙属被分析了。还对15个属于4个科的雀形目物种进行了比较研究（达尔文地雀科，Thrupidae，Icteridae，Emberizidae）。结果显示其长度为1202 bp，可分为三部分：结构域I，结构域II和结构域III。遗传距离显示不同科的平均差异为0.36％（达尔文地面雀科）至9.20％（Emberizidae），表明达尔文地面雀科可能是非常保守的单源物种。还发现了一些特定的序列片段，例如保守序列框，框C，D，F和E，HSP / LSP，CSB-1，TAS-1 。，而在其中找不到B-box，CSB-2和CSB-3。在所有选定的15个物种中均检测到F-box，其长度为29 bp，无变异位点。在E-box中，21个核苷酸位置中的20个与所选物种中的保守位置相同。D-box中有两个变体站点，C-box中有五个变体站点。以系统树为基础，可以清楚地看到，在所有选择的科中，大型木犀科（G. magnirostris）与科的科的关系最为紧密。通过这项研究，探索和了解了虎耳草控制区的结构特征。这项研究为深入研究虎杖提供了更多的基础信息。。这也提醒我们，我们应该更加注意探索达尔文雀的控制区域的特征。