Gram-negative bacteria release nanovesicles, called outer membrane vesicles (OMVs), from their outer membrane. Proteomics has been used to determine their composition. OMVs contain proteins able to elicit an immune response, so they have been proposed as a model to develop acellular vaccines. In this study, OMVs of Brucella suis, B. ovis, B. canis, and B. neotomae were purified and analyzed by SDS-PAGE, transmission electron microscopy and liquid chromatography coupled to mass spectrometry to determine the pan-proteome of these vesicles. In addition, antigenic proteins were detected by western blot with anti-Brucella sera. The in silico analysis of the pan-proteome revealed many homologous proteins, such as Omp16, Omp25, Omp31, SodC, Omp2a, and BhuA. Proteins contained in the vesicles from different Brucella species were detected by anti-Brucella sera. The occurrence of previously described immunogenic proteins derived from OMVs supports the use of these vesicles as candidates to be evaluated as an acellular brucellosis vaccine. Supplementary Information The online version contains supplementary material available at 10.1007/s00203-020-02170-w.

中文翻译：

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猪布鲁氏菌、绵羊布鲁氏菌、犬布鲁氏菌和新生布鲁氏菌外膜囊泡的蛋白质组学比较分析

革兰氏阴性细菌从它们的外膜释放纳米囊泡，称为外膜囊泡 (OMV)。蛋白质组学已被用于确定它们的组成。OMV 含有能够引发免疫反应的蛋白质，因此它们已被提议作为开发无细胞疫苗的模型。在本研究中，我们对猪布鲁氏菌、绵羊双歧杆菌、犬双歧杆菌和新芽孢杆菌的 OMV 进行了纯化，并通过 SDS-PAGE、透射电子显微镜和液相色谱与质谱联用进行了分析，以确定这些囊泡的泛蛋白质组。此外，用抗布鲁氏菌血清通过蛋白质印迹检测抗原蛋白。泛蛋白质组的计算机分析揭示了许多同源蛋白质，例如 Omp16、Omp25、Omp31、SodC、Omp2a 和 BhuA。通过抗布鲁氏菌血清检测来自不同布鲁氏菌属物种的囊泡中所含的蛋白质。先前描述的源自 OMV 的免疫原性蛋白的出现支持将这些囊泡用作候选物以作为无细胞布鲁氏菌病疫苗进行评估。补充信息 在线版本包含可从 10.1007/s00203-020-02170-w 获得的补充材料。