Abstract

Parallel fibrous scaffolds play a critical role in controlling the morphology of cells to be more natural and biologically inspired. Among popular tissue engineering materials, poly(2-hydroxyethyl methacrylate) (pHEMA) has been widely investigated in conventional forms due to its biocompatibility, low toxicity, and hydrophilicity. However, the swelling of pHEMA in water remains a major concern. To address this issue, randomly oriented and aligned as-spun pHEMA nanofibrous scaffolds were first fabricated at speeds of 300 and 2000 rpm in this study, which were then post-treated using either a thermal or a freeze-drying method. In cell assays, human dermal fibroblasts (HDFs) adhered to the freeze-drying treated substrates at a significantly faster rate, whereas they had a higher cell growth rate on thermally-treated substrates. Results indicated that the structural properties of pHEMA nanofibrous scaffolds and subsequent cellular behaviors were largely dependent on post-treatment methods. Moreover, this study suggests that aligned pHEMA nanofibrous substrates tended to induce regular fibroblast orientation and unidirectionally oriented actin cytoskeletons over random pHEMA nanofibrous substrates. Such information has predictive power and provides insights into promising post-treatment methods for improving the properties of aligned pHEMA scaffolds for numerous tissue engineering applications.

Graphic abstract

中文翻译：

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通过对齐和电纺纳米纤维膜的热和冷冻干燥后处理增强的多功能成纤维细胞。

摘要

平行纤维支架在控制细胞形态变得更加自然和生物学启发方面起着至关重要的作用。在流行的组织工程材料中，聚甲基丙烯酸2-羟乙酯（pHEMA）具有生物相容性，低毒性和亲水性等优点，因此以常规形式进行了广泛研究。但是，pHEMA在水中的溶胀仍然是一个主要问题。为了解决这个问题，在这项研究中，首先以300和2000 rpm的速度制造了随机取向和排列的纺丝pHEMA纳米纤维支架，然后使用热或冷冻干燥方法对其进行后处理。在细胞分析中，人真皮成纤维细胞（HDF）以明显较快的速率粘附于经冷冻干燥处理的底物，而它们在经过热处理的底物上具有较高的细胞生长速率。结果表明，pHEMA纳米纤维支架的结构特性和随后的细胞行为在很大程度上取决于后处理方法。此外，这项研究表明，对齐的pHEMA纳米纤维底物倾向于在随机的pHEMA纳米纤维底物上诱导规则的成纤维细胞取向和单向取向的肌动蛋白细胞骨架。此类信息具有预测能力，并为有前途的后处理方法提供了见识，这些后处理方法可为许多组织工程应用改善对齐的pHEMA支架的性能。这项研究表明，比起随机的pHEMA纳米纤维基质，对齐的pHEMA纳米纤维基质易于诱导规则的成纤维细胞取向和单向取向的肌动蛋白细胞骨架。此类信息具有预测力，并为有前途的后处理方法提供了见识，这些方法可为许多组织工程应用改善对齐的pHEMA支架的性能。这项研究表明，比起随机的pHEMA纳米纤维基质，对齐的pHEMA纳米纤维基质易于诱导规则的成纤维细胞取向和单向取向的肌动蛋白细胞骨架。此类信息具有预测能力，并为有前途的后处理方法提供了见识，这些后处理方法可为许多组织工程应用改善对齐的pHEMA支架的性能。

图形摘要