Feline bocavirus 1 (FBoV-1) may be associated with diarrhea in cats. In this study, a SYBR Green I-based quantitative polymerase chain reaction (qPCR) assay was established to detect FBoV-1. The melting curve showed a single melting peak at 83.0 ℃. The results of sensitivity showed that the detection limit of the qPCR was 3.87 × 10¹ copies/μL. Of note, the detection limit of the conventional polymerase chain reaction (cPCR) was 3.87 × 10³ copies/μL. The highest intra-assay and inter-assay coefficients of variation (CV%) were 0.98% and 1.42%, respectively. The positive detection rate of 128 clinical samples using the qPCR and the cPCR was 7.0% (9/128) and 4.7% (6/128), respectively. Taken together, these results indicated that the established qPCR assay has good sensitivity, high specificity, and good reproducibility. Therefore, it could provide support for the rapid and efficient clinical detection of FBoV-1.

猫博卡病毒1（FBoV-1）可能与猫的腹泻有关。在这项研究中，建立了一种基于SYBR Green I的定量聚合酶链反应（qPCR）分析方法来检测FBoV-1。熔解曲线在83.0℃显示一个熔解峰。灵敏度结果表明，qPCR的检出限为3.87×10 ^1个 拷贝/μL。值得注意的是，常规聚合酶链反应（cPCR）的检测限为3.87×10 ³ 拷贝/μL。批内和批间变异系数（CV％）最高，分别为0.98％和1.42％。使用qPCR和cPCR对128个临床样品的阳性检出率分别为7.0％（9/128）和4.7％（6/128）。综上所述，这些结果表明建立的qPCR测定法具有良好的灵敏度，高特异性和良好的再现性。因此，它可为FBoV-1的快速有效临床检测提供支持。