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In vitro performance and in vivo fertility of antibiotic-free preserved boar semen stored at 5 °C
Journal of Animal Science and Biotechnology ( IF 7 ) Pub Date : 2021-01-11 , DOI: 10.1186/s40104-020-00530-6
Helen Jäkel , Kathi Scheinpflug , Kristin Mühldorfer , Rafael Gianluppi , Matheus Schardong Lucca , Ana Paula Gonçalves Mellagi , Fernando Pandolfo Bortolozzo , Dagmar Waberski

Hypothermic preservation of boar semen is considered a potential method for omitting antibiotics from insemination doses, thereby contributing to the global antibiotic resistance defence strategy. The main challenges are chilling injury to spermatozoa and bacterial growth during semen storage leading to reduced fertility. To examine chilling injury and the number and type of bacteria in boar semen stored at 5 °C in the absence of antibiotics, and to assess the applicability of hypothermic semen storage under field conditions. Boar ejaculates were extended with AndroStar® Premium, stored at 17 °C with and at 5 °C without antibiotics and tested for functional sperm parameters by flow cytometry. Raw semen and extended samples were investigated bacteriologically. Fertility was evaluated after once-daily inseminations of 194 sows in a field study. Lethal sperm damage assessed by motility and membrane integrity was low throughout storage in both experimental groups. Sublethal chilling effects based on the decrease of viable spermatozoa with low membrane fluidity were higher (P < 0.05) up until 72 h in sperm stored at 5 °C compared to 17 °C but did not differ after 144 h. After 72 h, incubation in capacitating medium for 60 min induced a similar decrease in viable sperm with high mitochondria membrane potential and low cytosolic calcium in both groups. In semen stored at 5 °C, bacteria counts were below 103 CFU/mL and the bacteria spectrum was similar to that of raw semen. In 88% of 34 boars, cooled semen fulfilled the requirements for insemination. Fertility was high and did not differ (P > 0.05) between sow groups inseminated with semen stored antibiotic-free at 5 °C and semen stored at 17 °C with antibiotics. Despite subtle chilling effects and low bacterial numbers, antibiotic-free hypothermic storage of boar semen offers the possibility to reduce the use of antibiotics in pig insemination. However, strict sanitary guidelines must be maintained and further evidence of efficiency under field conditions is considered desirable.

中文翻译:

无抗生素保存的公猪精液在5°C下的体外性能和体内受精性

公猪精液的低温保存被认为是从授精剂量中省略抗生素的一种潜在方法,从而为全球抗生素耐药性防御策略做出了贡献。主要挑战是精子受冷害和精液储存过程中细菌的生长,导致生育力下降。检查在不使用抗生素的情况下在5°C下储存的公猪精液的冷害以及细菌的数量和类型,并评估低温精液在野外条件下的适用性。用AndroStar®Premium扩展公猪射精,在没有抗生素的情况下在17°C和在5°C下存储,并通过流式细胞术测试功能性精子参数。生精液和扩展样品进行了细菌学研究。在田间研究中,每天对194只母猪进行每天一次的授精后,评估其生育力。通过运动性和膜完整性评估的致死性精子损害在两个实验组的整个存储过程中都很低。在5°C储存的精子中,直到72 h时,基于存活的低膜流动性的精子减少的亚致死冷却效果较高(P <0.05),而17°C则没有变化。72小时后,在容性培养基中温育60分钟,两组的活精子率都有相似的下降,线粒体膜电位高,胞质钙含量低。在5°C下储存的精液中,细菌计数低于103 CFU / mL,并且细菌光谱类似于未加工的精液。在34头公猪中,有88%的精液满足了人工授精的要求。生育率很高,没有差异(P> 0。05)在母猪组之间进行授精,授精的精液在5°C时不使用抗生素,而精液在17°C时用抗生素来保存。尽管有微妙的凉爽作用和低细菌数,但公猪精液中无抗生素的低温保存仍为减少猪授精中抗生素的使用提供了可能。但是,必须遵守严格的卫生准则,并希望在野外条件下进一步证明效率。
更新日期:2021-01-11
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