From an animal production and health perspective, our understanding of the metabolites in ruminant biofluids, particularly rumen fluid across different host species is poorly understood. Metabolomics is a powerful and sensitive approach for investigating low molecular weight metabolite profiles present in rumen biofluids. It can be used to identify potential roles of metabolites in the rumen microbiome and provide and understanding of host-level regulatory mechanisms associated with animal production. The rumen is a strictly anaerobic environment enriched with a complex community of bacteria, protozoa, fungi, archaea and bacteriophages. Here, we present a metabolomic dataset generated using hydrophilic interaction liquid chromatography (HILIC) and semi-polar (C18) chromatography methods coupled to high resolution mass spectrometry (MS), collected in both positive and negative ionization modes, of ovine rumen samples that were fractionated based on molecular weight (20 kDa, 8-10 kDa and 100 Da). This study highlights the potential of HILIC and C18 chromatography combined with non-targeted mass spectrometric methods to detect the polar and semi-polar metabolite species of the ruminal fluid metabolome.

中文翻译：

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来自绵羊瘤胃液组分的非靶向多模式代谢组学数据

从动物生产和健康的角度来看，我们对反刍动物生物体液中代谢物的理解，尤其是不同宿主物种的瘤胃液中的代谢物知之甚少。代谢组学是一种强大而灵敏的方法，用于研究瘤胃生物流体中存在的低分子量代谢物概况。它可用于识别代谢物在瘤胃微生物组中的潜在作用，并提供和理解与动物生产相关的宿主水平调节机制。瘤胃是一个严格厌氧的环境，富含细菌、原生动物、真菌、古细菌和噬菌体的复杂群落。在这里，我们展示了使用亲水相互作用液相色谱 (HILIC) 和半极性 (C18) 色谱方法与高分辨率质谱 (MS) 耦合生成的代谢组学数据集，以正电离和负电离模式收集的羊瘤胃样品根据分子量（20 kDa、8-10 kDa 和 100 Da）进行分级。本研究强调了 HILIC 和 C18 色谱结合非靶向质谱方法检测瘤胃液代谢组的极性和半极性代谢物种类的潜力。