Exosomes have shown great potential in disease diagnostics and therapeutics. However, current isolation approaches are burdensome and suffer from low speed, yield and purity, limiting basic research and clinical applications. Here, we describe an efficient exosome detection method via the ultrafast-isolation system (EXODUS) that allows automated label-free purification of exosomes from varied biofluids. We obtained the ultra-efficient purification of exosomes by negative pressure oscillation and double coupled harmonic oscillator–enabled membrane vibration. Our two coupled oscillators generate dual-frequency transverse waves on the membranes, enabling EXODUS to outperform other isolation techniques in speed, purity and yield. We demonstrated EXODUS by purifying exosomes from urine samples of 113 patients and validated the practical relevance in exosomal RNA profiling with the high-resolution capability and high-throughput analysis.

外泌体在疾病诊断和治疗方面显示出巨大潜力。然而，目前的分离方法繁琐，速度慢，收率低，纯度低，限制了基础研究和临床应用。在这里，我们描述了一种通过超快分离系统 (EXODUS) 实现的有效外泌体检测方法，该方法允许从各种生物流体中自动无标记纯化外泌体。我们通过负压振荡和双耦合谐振子启用的膜振动获得了外泌体的超高效纯化。我们的两个耦合振荡器在膜上产生双频横波，使 EXODUS 在速度、纯度和产量方面优于其他隔离技术。