Abstract

Reference intervals (RIs), developed as part of the Nordic Reference Interval Project 2000 (NORIP) are widely used in most European laboratories. We aimed to examine the validity of the NORIP RIs by establishing RIs for 12 frequently used laboratory tests based on data from a local Danish population and compare these local RIs with the NORIP RIs. Using an a posteriori direct sampling approach, blood sample data were assessed from 11,138 participants aged 18+ years in the Lolland-Falster Health Study (LOFUS), of whom 2154 turned out to meet criteria for being healthy for inclusion in establishing RIs according to the NORIP methodology. The 2.5th and 97.5th percentiles were calculated for alanine aminotransferase (ALAT), albumin, alkaline phosphatase, bilirubin, creatinine, hemoglobin, high-density lipoprotein cholesterol, iron, low-density lipoprotein cholesterol, thrombocytes, total cholesterol, and triglycerides. When comparing our estimates with the NORIP, the lower reference limits (RLs) for bilirubin and iron were lower, and higher for ALAT, thrombocytes and triglycerides. Upper RLs were lower for albumin (males and females ≥70 years), bilirubin and iron, but higher for alkaline phosphatase, triglycerides and for creatinine in men. In LOFUS, approximately 20% of the participants were healthy and qualified for inclusion in the establishment of RIs. Several of the local RIs differed from the NORIP RIs.

摘要

作为北欧参考间隔项目2000（NORIP）的一部分开发的参考间隔（RI）在大多数欧洲实验室中得到了广泛使用。我们旨在通过基于丹麦当地人口的数据建立12种常用实验室测试的RI，以检验NORIP RI的有效性，并将这些本地RI与NORIP RI进行比较。使用后验直接抽样法，在Lolland-Falster健康研究（LOFUS）中从11138名18岁以上的参与者中评估了血液样本数据，其中2154人符合根据NORIP方法纳入建立RI的健康标准。计算了谷丙转氨酶（ALAT），白蛋白，碱性磷酸酶，胆红素，肌酐，血红蛋白，高密度脂蛋白胆固醇，铁，低密度脂蛋白胆固醇，血小板，总胆固醇和甘油三酸酯的第2.5和97.5个百分位。将我们的估计值与NORIP进行比较时，胆红素和铁的参考下限（RLs）较低，而ALAT，血小板和甘油三酸酯的参考下限较低。白蛋白（70岁及以上的男性和女性），胆红素和铁的较高RLs较低，而碱性磷酸酶的较高RLs较高，甘油三酸酯和肌酐的男性。在LOFUS中，约有20％的参与者健康并且符合纳入RI的资格。几个本地RI与NORIP RI不同。