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Identification of novel single-nucleotide polymorphism at exon1 and 2 region of B4GALT1 gene and its association with milk production traits in crossbred cattle of Kerala, India
Animal Biotechnology ( IF 3.7 ) Pub Date : 2021-01-10
Jamuna Valsalan, Tina Sadan, Thirupathy Venkatachalapathy, Kulangara Anilkumar, Thazhathu Veettil Aravindakshan

Abstract

Beta 1,4-galactosyltransferase-I gene (B4GALT1) is an important candidate gene for milk performance traits, encodes catalytic part of lactose synthesis. Main objectives of present study is identification of single-nucleotide polymorphism in exon 1 and 2 region of B4GALT1 and to find significant association of genetic variants with milk performance traits in crossbred cattle of Kerala. The study was conducted on two hundred crossbred cattle maintained at various farms of Kerala Veterinary and Animal Sciences University, India. Genomic DNA was isolated and polymorphism of gene were detected by Single Strand Confirmation Polymorphism. Genotype and allelic frequency were estimated. Chi-square analysis revealed that screened population is under Hardy Weinberg equilibrium. Nucleotide sequence analysis revealed a novel non-synonymous single-nucleotide variation (T94A) in exon 1 and a non-synonymous mutation of T97C in exon 2 of B4GALT1 gene in the screened cattle population. Association analysis of genetic variants was done with milk production traits and major non-genetic factors using fixed models. Different genetic variants of B4GALT1 was significantly associated with 305 days milk yield, lactose, protein percent. Study indicates existence of genetic variability in B4GALT1 gene on crossbred cattle of Kerala and suggests a scope of considering genetic variants of B4GALT1in selection strategies.



中文翻译:

印度喀拉拉邦杂交牛B4GALT1基因外显子1和2区新型单核苷酸多态性的鉴定及其与产奶性状的关系

摘要

Beta 1,4-半乳糖基转移酶-I基因(B4GALT1)是牛奶性能特征的重要候选基因,编码乳糖合成的催化部分。本研究的主要目的是鉴定B4GALT1外显子1和2区域的单核苷酸多态性。并发现喀拉拉邦杂交牛的遗传变异与牛奶生产性状之间的显着关联。这项研究是在印度喀拉拉邦兽医和动物科学大学各个农场饲养的200头杂交牛上进行的。分离基因组DNA,并通过单链确认多态性检测基因的多态性。估计基因型和等位基因频率。卡方分析表明,筛选出的种群处于Hardy Weinberg平衡之下。核苷酸序列分析揭示在外显子1的新颖的非同义的单核苷酸变异(T94A)和T97C的外显子2的非同义突变B4GALT1被筛选的牛群中的基因。使用固定模型对牛奶生产性状和主要的非遗传因素进行了遗传变异的关联分析。B4GALT1的不同遗传变异与305天的产奶量,乳糖,蛋白质百分比显着相关。研究表明喀拉拉邦杂交牛的B4GALT1基因存在遗传变异,并提出了在选择策略中考虑B4GALT1遗传变异的范围。

更新日期:2021-01-11
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