ABSTRACT

Calcareous soil may limit plant growth depending upon plant nutrient uptake. The change of apoplastic pH and deficiency of nutrients in high lime soils negatively affects plant growth with chlorophyll synthesis, root growth, enzyme synthesis. To overcome this abiotic stress, control (non-inoculated) and six bacterial strains; Alcaligenes 637Ca, Agrobacterium A18, Staphylococcus MFDCa1, Staphylococcus MFDCa2, Bacillus M3, and Pantoea FF1 were inoculated one-year-old apple saplings grafted on M9 and MM106. The M3, MFDCa1, FF1, and MFDCa2 strains were found to have the largest leaf area on M9 rootstock, while 637Ca had the smallest leaf area on MM106 rootstock. In the stem diameter, A18 and MFDCa1 were measured lower in M9 rootstock, while MFDCa1 and control were found lower in MM106 rootstock. The shortest shoot was determined in MFDCa2 on MM106 rootstock, while the FF1, M3, and control were found tallest shoot on M9 rootstock. For plant nutrition, all bacterial treatments were found higher than the control in both macronutrients and micronutrients. While the content of the N, P, Ca, and Cu was found highest in MM106 rootstock, K, Mg, Mn, Zn, and B were determined the highest on M9 rootstock. The FF1 and M3 bacteria strains were commonly determined to have the highest contents of the N, P, K, Mg, and Ca on M9 rootstock. As a result, bacterial strains used in the present study have an important potential to increase plant growth and to be used as a biofertilizer for Braeburn apple cultivar on M9 and MM106 under lime soil conditions.

摘要

钙质土壤可能会限制植物的生长，具体取决于植物吸收养分的情况。高石灰土壤中质外性pH的变化和养分的缺乏对叶绿素合成，根系生长和酶合成的植物生长产生负面影响。为了克服这种非生物胁迫，应控制（未接种）和六个细菌菌株。产碱菌637Ca，农杆菌A18，葡萄球菌MFDCa1，葡萄球菌MFDCa2，芽孢杆菌M3和泛菌FF1接种了嫁接在M9和MM106上的一岁苹果幼树。发现M3，MFDCa1，FF1和MFDCa2菌株在M9砧木上具有最大的叶面积，而637Ca在MM106砧木上具有最小的叶面积。在茎直径中，M9砧木的A18和MFDCa1含量较低，而MM106砧木的MFDCa1和对照含量较低。在MF106砧木上的MFDCa2中确定了最短的芽，而在M9砧木上发现了FF1，M3和对照的最高芽。对于植物营养，在常量营养素和微量营养素中，所有细菌处理均高于对照。在MM106砧木中发现N，P，Ca和Cu的含量最高，而在M9砧木中发现K，Mg，Mn，Zn和B的含量最高。通常确定FF1和M3细菌菌株在M9砧木上具有最高的N，P，K，Mg和Ca含量。结果，本研究中使用的细菌菌株具有提高植物生长的重要潜力，并在石灰土壤条件下用作M9和MM106上Braeburn苹果品种的生物肥料。