Oxidative stress inevitably occurs during oocyte maturation in vitro. α‐lipoic acid (α‐LA) has a strong antioxidant capacity, but the effect of α‐LA on parthenogenetic activation of oocytes was rarely reported. This study aims to investigate the effect of supplementing α‐LA to in vitro maturation medium on the subsequent developmental ability of goat parthenogenetic embryos during oocytes maturation. In the study, the goat cumulus‐oocyte complex was divided into the experimental (with 25 μmol/L α‐LA) and the control (without α‐LA) groups. Oxidase expression was measured using RT‐qPCR. After 18–22 hr of maturation, the oocytes were then parthenogenetic activated. The total antioxidant capacity of embryos was measured after 0, 24, 48, 72 and 96 hr of culture. Rates of oocyte maturation and the rates of development for parthenogenetic embryos in the α‐LA group were significantly improved by 7.88% (p < .05) and 5.41% (p < .05) compared with those in the control group, respectively. After 24 hr, the difference in total antioxidant capacity was extremely significant in both groups. An evident decrease in the control group and a minor decrease in the α‐LA group were observed (p < .01). The ratio of inner cell mass cells to the total cell number of blastocysts in the α‐LA group increased compared with that in the control group (p < .05) on day 8. α‐LA significantly promoted the expression of SOD and GPX4 of parthenogenetic blastocysts and maturated oocytes. α‐LA (25 μmol/L) improved the maturation rate and the developmental competence of the parthenogenetic activation of oocytes, which might be mediated by maintaining the total antioxidant ability of oocytes during the culture period.

中文翻译：

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α-硫辛酸提高体外山羊卵母细胞的成熟度和发育潜力

在体外卵母细胞成熟过程中不可避免地会发生氧化应激。α-硫辛酸（α-LA）具有很强的抗氧化能力，但很少报道α-LA对卵母细胞孤雌生殖激活的影响。本研究旨在研究在体外成熟培养基中补充α-LA对卵母细胞成熟过程中山羊孤雌生殖胚胎后续发育能力的影响。在研究中，山羊卵丘-卵母细胞复合物分为实验组（含 25 μmol/L α-LA）和对照组（不含 α-LA）。使用 RT-qPCR 测量氧化酶表达。成熟 18-22 小时后，卵母细胞被孤雌生殖激活。在培养 0、24、48、72 和 96 小时后测量胚胎的总抗氧化能力。p  < .05) 和 5.41% ( p  < .05) 分别与对照组相比。24 小时后，两组的总抗氧化能力差异非常显着。观察到对照组的明显减少和 α-LA 组的轻微减少 ( p  < .01)。第8天α-LA组内细胞团细胞占囊胚总细胞数的比例较对照组增加（p  < .05）。α-LA显着促进SOD和GPX4的表达孤雌生殖囊胚和成熟卵母细胞。α-LA (25 μmol/L) 提高了卵母细胞孤雌生殖激活的成熟率和发育能力，这可能是通过在培养期间维持卵母细胞的总抗氧化能力来介导的。