Introduction

The success of pregnancy depends on the regulation of immunological processes in the placenta. Important mediators of an immune response include pro- and anti-inflammatory interleukins which may be regulated by CYP epoxygenases and their metabolites. The relation between interleukins and CYP epoxygenases expression in human placenta has not yet been studied vastly.

Material and methods

We investigated the expression patterns of IL-1β and IL-10 in embryonic (n = 8), early foetal (n = 16) and term (n = 7) human placenta tissue by an immunohistochemical method and evaluated the results by Kruskal-Wallis test. The obtained data was correlated using Spearman’s correlation coefficient to our previously published data of CYP epoxygenases expression in the same samples. To confirm that Hofbauer cells express IL-10 and IL-1β as well as CYP2C8 and IL-10 together, and thus there is a relation between proteins of interests, we used multiplex immunofluorescent staining.

Results

The expression of IL-1β decreased with gestational age in cytotrophoblast, syncytiotrophoblast, as well as in Hofbauer cells whilst IL-10 decreased in cytotrophoblast, remained at the same levels in syncytiotrophoblast and increased in Hofbauer cells. In trophoblast cells, we found a statistically significant positive correlation between the expression of CYP2J2 and CYP2C9 with IL-1β, whereas there was no relation between IL-10 and any of the tested CYP epoxygenases. In Hofbauer cells, we found a significant positive correlation between CYP2C8 and IL-10 and a significant negative correlation between CYP2C8 and IL-1β.

Conclusion

Our results showed that the exact role and relation of interleukins and CYP epoxygenases and their metabolites is dependent on their respective cellular context. Because of IL-10, IL-1β, as well as HBCs play a role in various pathological conditions, further investigation of the exact role of CYP epoxygenase, interleukins and their relations is needed.

中文翻译：

---

正常人胎盘中IL-1β和IL-10的表达模式及其与CYP环氧化酶的关系

介绍

怀孕的成功取决于胎盘中免疫过程的调节。免疫反应的重要介质包括促炎和抗炎白介素，它们可能受 CYP 环氧化酶及其代谢物的调节。人胎盘中白细胞介素和 CYP 环氧化酶表达之间的关系尚未得到广泛研究。

材料与方法

我们通过免疫组织化学方法研究了 IL-1β 和 IL-10 在胚胎 (n  =  8)、早期胎儿 (n  =  16) 和足月 (n  =  7) 人胎盘组织中的表达模式，并通过 Kruskal-Wallis 评估了结果测试。使用 Spearman 相关系数将获得的数据与我们之前公布的相同样品中 CYP 环氧化酶表达数据相关联。为了确认 Hofbauer 细胞同时表达 IL-10 和 IL-1β 以及 CYP2C8 和 IL-10，从而在感兴趣的蛋白质之间存在关系，我们使用了多重免疫荧光染色。

结果

IL-1β 在细胞滋养层、合体滋养层和 Hofbauer 细胞中的表达随着孕龄而降低，而细胞滋养层中 IL-10 的表达降低，在合体滋养层中保持相同水平，而在 Hofbauer 细胞中则增加。在滋养层细胞中，我们发现 CYP2J2 和 CYP2C9 的表达与 IL-1β 之间存在统计学上显着的正相关，而 IL-10 与任何测试的 CYP 加氧酶之间没有相关性。在霍夫鲍尔细胞中，我们发现 CYP2C8 和 IL-10 之间显着正相关，CYP2C8 和 IL-1β 之间显着负相关。

结论

我们的结果表明，白细胞介素和 CYP 环氧化酶及其代谢物的确切作用和关系取决于它们各自的细胞环境。由于 IL-10、IL-1β 以及 HBCs 在各种病理条件下发挥作用，需要进一步研究 CYP 加氧酶、白细胞介素及其关系的确切作用。