Helminths can interact with their hosts in many different ways, including through the secretion of soluble molecules (such as lipids, glycans and proteins) and extracellular vesicles (EVs). The field of helminth secreted EVs has significantly advanced in recent years, mainly due to the molecular characterisation of EV proteomes and research highlighting the potential of EVs and their constituent molecules in the diagnosis and control of parasitic infections. Despite these advancements, the lack of appropriate isolation and purification methods is impeding the discovery of suitable biomarkers for the differentiation of helminth EV populations. In the present review we offer our viewpoint on the different proteomic techniques and approaches that have been developed, as well as solutions to common pitfalls and challenges that could be applied to advance the study of helminth EVs.

蠕虫可以通过许多不同的方式与其宿主相互作用，包括通过分泌可溶性分子（例如脂质，聚糖和蛋白质）和细胞外囊泡（EV）。近年来，蠕虫分泌型EV的领域有了显着发展，这主要是由于EV蛋白质组的分子表征以及研究突出了EV及其组成分子在诊断和控制寄生虫感染方面的潜力。尽管取得了这些进步，但缺乏合适的分离和纯化方法仍阻碍了用于区分蠕虫EV种群的合适生物标记物的发现。在本综述中，我们对已开发出的不同蛋白质组学技术和方法提出了自己的观点，