Biomass alkaline deacetylation prior to acid pretreatment can be a promising alternative to reduce the toxicity of hemicellulosic hydrolysates and improve second-generation bioethanol production. In this paper, the effect of alkaline deacetylation of sugarcane bagasse on bioethanol production by Spathaspora passalidarum was evaluated. Sugarcane bagasse deacetylated hemicellulosic hydrolysate (DHH) was processed using the following sequence: (1) deacetylation (0.4%, w/v NaOH, 70 °C, 3 h) and (2) acid pretreatment (0.5% (v/v) of H₂SO₄, 140 °C, 15 min). Non-deacetylated, hemicellulosic hydrolysate (HH), was obtained applying only acid pretreatment (0.5% (v/v) of H₂SO₄, 140 °C, 15 min). Biomass deacetylation reduced the content of acetic acid and some phenolic compounds in the hydrolysate (DHH) compared to acid pretreatment (HH), which resulted in its low toxicity. Thus, the bioethanol production with DHH was of 16.92 g L⁻¹, whereas only 1.3 g L⁻¹ of bioethanol was obtained with HH fermentation. Deacetylation process provided a 13-fold increase in bioethanol production by S. passalidarum, showing that alkaline deacetylation followed by sulfuric acid pretreatment is a promising strategy to increase bioethanol production. This procedure provided a simple and practical alternative to the classic methods of detoxification of hemicellulosic hydrolysate from sugarcane bagasse.

酸预处理之前的生物质碱性脱乙酰作用可能是减少半纤维素水解产物毒性并提高第二代生物乙醇产量的有前途的替代方法。本文评价了甘蔗渣的碱性脱乙酰作用对Passpathida Srumaspora passalidarum生产生物乙醇的影响。甘蔗渣脱乙酰半纤维素水解物（DHH）按以下顺序进行处理：（1）脱乙酰（0.4％，w / v NaOH，70°C，3 h）和（2）酸预处理（0.5％（v / v） ħ ₂ SO ₄，140℃，15分钟）。仅通过酸预处理（0.5％（v / v）的H ₂ SO ₄）获得未脱乙酰化的半纤维素水解物（HH），140℃，15分钟）。与酸预处理（HH）相比，生物质脱乙酰化可降低水解产物（DHH）中乙酸和某些酚类化合物的含量，从而降低了毒性。因此，用DHH生产的生物乙醇为16.92g L ^-1，而通过HH发酵仅获得1.3g L ^-1的生物乙醇。脱乙酰过程使Passalidarum的生物乙醇产量增加了13倍，这表明碱性脱乙酰后进行硫酸预处理是增加生物乙醇产量的有前途的策略。该方法为从甘蔗渣中解毒半纤维素水解产物的经典方法提供了一种简单实用的替代方法。