Abstract

It is of great significance to establish sensitive and accurate pathogen detection methods, considering the continuous emergence or re-emergence of infectious diseases seriously influences the safety of human and animals. Proximity ligation assay (PLA) is developed for the sensitive protein detection and also can be used for the detection of pathogens. PLA employs aptamer or monoclonal/polyclonal antibody-nucleic acid complexes as proximity probes. When the paired proximity probes bind to the same target protein or protein complex, they will be adjacent to each other and form an amplifiable DNA sequence through ligation. Combining the specificity of enzyme-linked immunosorbent assay (ELISA) and sensitivity of polymerase chain reaction (PCR), PLA transforms the detection of protein into the detection of DNA nucleic acid sequence. Therefore, as an ultrasensitive protein assay, PLA has great potential for quantification, localization of protein, and clinical diagnostics. In this review, we summarize the basic principles of PLA and its applications in pathogen detection.

Key Points

• Different forms of proximity ligation assay are introduced.

• Applications of proximity ligation assay in pathogen detection are summarized.

• Proximity ligation assay is an ultrasensitive method to quantify protein and pathogen.

中文翻译：

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邻近结扎测定：一种超灵敏的蛋白质定量方法及其在病原体检测中的应用

摘要

考虑到传染病的不断出现或再出现严重影响人畜的安全，建立灵敏，准确的病原体检测方法具有重要意义。邻近连接测定法（PLA）是为检测敏感蛋白而开发的，还可用于病原体的检测。PLA使用适体或单克隆/多克隆抗体-核酸复合物作为邻近探针。当配对的邻近探针与相同的目标蛋白质或蛋白质复合物结合时，它们将彼此相邻并通过连接形成可扩增的DNA序列。结合酶联免疫吸附测定（ELISA）的特异性和聚合酶链反应（PCR）的灵敏度，PLA将蛋白质的检测转换为DNA核酸序列的检测。因此，作为一种超灵敏的蛋白质测定法，PLA在定量，蛋白质定位和临床诊断方面具有巨大的潜力。在这篇综述中，我们总结了PLA的基本原理及其在病原体检测中的应用。

关键点

•介绍了不同形式的邻近连接测定法。

•总结了邻近结扎法在病原体检测中的应用。

•邻近连接测定法是定量蛋白质和病原体的超灵敏方法。