Glycans are responsible for many biological activities; however, their structures are incredibly diverse and complex, often rendering the field of glycomics unsolvable by a single analytical technique. The development of multiple chemical derivatization strategies and bioinformatic software is responsible for some of the greatest analytical gains in the field of glycomics. The INLIGHT strategy is a chemical derivatization technique using hydrazide chemistry to derivatize the reducing end of N-linked glycans and incorporates either a natural (NAT, ¹²C₆) or a stable-isotope label (SIL, ¹³C₆) to carry out relative quantification. Here we present GlycoHunter, a user-friendly software created in MATLAB that enables researchers to accurately and efficiently process MS1 glycomics data where a NAT and SIL pair is generated for relative quantification, including but not limited to, INLIGHT. GlycoHunter accepts the commonly used data file formats imzML and mzXML and effectively identifies all peak pairs associated with NAT- and SIL-labeled N-linked glycans using MS1 data. It also includes the ability to tailor the search parameters and export the results for further analysis using Skyline or Excel.

中文翻译：

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GlycoHunter：用于检测和相对定量 INLIGHT 标记的 N-连接聚糖的开源软件

聚糖负责许多生物活动；然而，它们的结构非常多样化和复杂，通常使糖组学领域无法通过单一的分析技术解决。多种化学衍生化策略和生物信息学软件的开发促成了糖组学领域的一些最大分析成果。INLIGHT 策略是一种化学衍生技术，使用酰肼化学来衍生 N-连接聚糖的还原末端，并结合天然（NAT，¹² C ₆）或稳定同位素标记（SIL，¹³ C ₆) 进行相对量化。在这里，我们介绍 GlycoHunter，这是一个在 MATLAB 中创建的用户友好型软件，使研究人员能够准确有效地处理 MS1 糖组学数据，其中生成 NAT 和 SIL 对以进行相对量化，包括但不限于 INLIGHT。GlycoHunter 接受常用的数据文件格式 imzML 和 mzXML，并使用 MS1 数据有效识别与 NAT 和 SIL 标记的 N 连接聚糖相关的所有峰对。它还包括定制搜索参数和导出结果以使用 Skyline 或 Excel 进行进一步分析的能力。