Identifying the effect of vancomycin on health care–associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media,GigaScience

当前位置： X-MOL 学术 › Gigascience › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Identifying the effect of vancomycin on health care–associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media
GigaScience ( IF 9.2 ) Pub Date : 2021-01-14 , DOI: 10.1093/gigascience/giaa156
Akanksha Rajput ₁ , Saugat Poudel ₁ , Hannah Tsunemoto ₂ , Michael Meehan _{3,

4} , Richard Szubin ₁ , Connor A Olson ₁ , Yara Seif ₁ , Anne Lamsa ₂ , Nicholas Dillon _{5,

6} , Alison Vrbanac _{5,

6} , Joseph Sugie ₂ , Samira Dahesh _{5,

6} , Jonathan M Monk ₁ , Pieter C Dorrestein _{3,

4,

7,

8} , Rob Knight _{1,

5,

8,

9} , Joe Pogliano ₂ , Victor Nizet _{4,

5,

6,

8} , Adam M Feist _{1,

10} , Bernhard O Palsson _{1,

5,

8,

10}

Affiliation

Department of Bioengineering, University of California, 9500 Gilman Dr, La Jolla, CA 92093, USA.
Division of Biological Sciences, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
Collaborative Mass Spectrometry Innovation Center, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
Department of Pediatrics, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92023, USA.
Collaborative to Halt Antibiotic-Resistant Microbes (CHARM), Department of Pediatrics, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
Center for Microbiome Innovation, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
Department of Computer Science and Engineering, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800 Kongens, Lyngby, Denmark.

Background The evolving antibiotic-resistant behavior of health care–associated methicillin-resistant Staphylococcus aureus (HA-MRSA) USA100 strains are of major concern. They are resistant to a broad class of antibiotics such as macrolides, aminoglycosides, fluoroquinolones, and many more. Findings The selection of appropriate antibiotic susceptibility examination media is very important. Thus, we use bacteriological (cation-adjusted Mueller-Hinton broth) as well as physiological (R10LB) media to determine the effect of vancomycin on USA100 strains. The study includes the profiling behavior of HA-MRSA USA100 D592 and D712 strains in the presence of vancomycin through various high-throughput assays. The US100 D592 and D712 strains were characterized at sub-inhibitory concentrations through growth curves, RNA sequencing, bacterial cytological profiling, and exo-metabolomics high throughput experiments. Conclusions The study reveals the vancomycin resistance behavior of HA-MRSA USA100 strains in dual media conditions using wide-ranging experiments.

中文翻译：

使用细菌学和生理学介质确定万古霉素对医疗保健相关的耐甲氧西林金黄色葡萄球菌菌株的影响

背景与医疗保健相关的耐甲氧西林金黄色葡萄球菌 (HA-MRSA) USA100 菌株不断演变的抗生素耐药行为备受关注。它们对大环内酯类、氨基糖苷类、氟喹诺酮类等多种抗生素具有抗药性。结果选择合适的抗生素敏感性检查培养基非常重要。因此，我们使用细菌学（阳离子调整的 Mueller-Hinton 肉汤）以及生理学（R10LB）培养基来确定万古霉素对 USA100 菌株的影响。该研究包括通过各种高通量测定分析 HA-MRSA USA100 D592 和 D712 菌株在万古霉素存在下的行为分析。US100 D592 和 D712 菌株通过生长曲线、RNA 测序、细菌细胞学分析和外代谢组学高通量实验。结论该研究通过广泛的实验揭示了 HA-MRSA USA100 菌株在双培养基条件下的万古霉素抗性行为。

更新日期：2021-01-14

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南

全部期刊列表>>