Interactions between climate change and ultraviolet radiation (UVR) have a substantial impact on aquatic ecosystems, especially on photosynthetic organisms. To counteract the damaging effects of UVR, cyanobacteria developed adaptive strategies such as the biosynthesis of secondary metabolites. This study aimed to evaluate the effects of UVR on the metabolomic profiles of potentially toxic cyanobacteria. Twelve strains were irradiated with ultraviolet A and ultraviolet B radiation and parabolic aluminized reflector lamps for 3 days, followed by liquid chromatography–tandem mass spectometry (LC-MS/MS) analysis to assess changes in metabolomic profiles. Matrices were used to generate principal component analysis biplots, and molecular networks were obtained using the Global Natural Products platform. Most strains showed significant changes in their metabolomic profiles after UVR exposure. On average, 7% of MS features were shown to be exclusive to metabolomic profiles before UVR exposure, while 9% were unique to metabolomic profiles after UVR exposure. The identified compounds included aeruginosins, spumigins, cyanopeptolins, microginins, namalides, pseudospumigins, anabaenopeptins, mycosporine-like amino acids, nodularins and microcystins. Data showed that cyanobacteria display broad metabolic plasticity upon UVR exposure, including the synthesis and differential expression of a variety of secondary metabolites. This could result in a competitive advantage, supporting cyanobacterial blooms under various UVR light exposures.

中文翻译：

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紫外线对潜在毒性蓝细菌代谢组学谱的影响

气候变化与紫外线辐射（UVR）之间的相互作用对水​​生生态系统，特别是对光合生物具有重大影响。为了抵消UVR的破坏作用，蓝细菌开发了适应性策略，例如次级代谢产物的生物合成。这项研究旨在评估UVR对潜在毒性蓝细菌代谢组学谱的影响。用紫外线A和紫外线B辐射和抛物线镀铝反射灯照射12个菌株3天，然后进行液相色谱-串联质谱分析（LC-MS / MS）以评估代谢组学谱的变化。矩阵用于生成主成分分析双峰，并使用Global Natural Products平台获得分子网络。大部分菌株在暴露于UVR后显示出其代谢组学特征的显着变化。平均而言，有7％的MS特征被UVR暴露之前的代谢组学特征所独有，而9％仅是UVR暴露之后的代谢组学特征所独有的。鉴定出的化合物包括铜绿素酶，spumigins，氰肽素，微ginins，纳马利德斯，假spumigins，anabaenopeptins，霉菌素样氨基酸，结节蛋白和微囊藻毒素。数据表明，蓝细菌在暴露于UVR后显示出广泛的代谢可塑性，包括多种次生代谢产物的合成和差异表达。这可能会产生竞争优势，从而在各种UVR光照下支持蓝藻绽放。已显示7％的MS功能是UVR暴露之前代谢组学特征所独有的，而9％是UVR暴露之后代谢组学特征所独有的。鉴定出的化合物包括铜绿素酶，spumigins，氰肽素，微ginins，纳马利德斯，假spumigins，anabaenopeptins，霉菌素样氨基酸，结节蛋白和微囊藻毒素。数据显示，蓝细菌在暴露于UVR后显示出广泛的代谢可塑性，包括多种次生代谢产物的合成和差异表达。这可能会产生竞争优势，从而在各种UVR光照下支持蓝藻绽放。已显示7％的MS功能是UVR暴露之前代谢组学特征所独有的，而9％是UVR暴露之后代谢组学特征所独有的。鉴定出的化合物包括铜绿素酶，spumigins，氰肽素，微ginins，纳马利德斯，假spumigins，anabaenopeptins，霉菌素样氨基酸，结节蛋白和微囊藻毒素。数据显示，蓝细菌在暴露于UVR后显示出广泛的代谢可塑性，包括多种次生代谢产物的合成和差异表达。这可能会产生竞争优势，从而在各种UVR光照下支持蓝藻绽放。霉菌素样氨基酸，结节蛋白和微囊藻毒素。数据显示，蓝细菌在暴露于UVR后显示出广泛的代谢可塑性，包括多种次生代谢产物的合成和差异表达。这可能会产生竞争优势，从而在各种UVR光照下支持蓝藻绽放。霉菌素样氨基酸，结节蛋白和微囊藻毒素。数据显示，蓝细菌在暴露于UVR后显示出广泛的代谢可塑性，包括多种次生代谢产物的合成和差异表达。这可能会产生竞争优势，从而在各种UVR光照下支持蓝藻绽放。