Probiotics play an important role in health benefits on the host. However, they also possess potentials for infectivity or in situ toxin production; thus, requiring a comprehensive assessment of their safety. In this study, we report genomic characteristics of a newly isolated Lactobacillus casei IDCC 3451 from infant feces. Phenotypic assays based on enzyme activities and carbohydrate fermentation profiles represented metabolic features of the strain. Safety evaluation for antimicrobial resistance, biogenic amines production and cytotoxicity to a murine mouse model suggested its safe use as a probiotic strain. Our findings on the genetic background of L. casei IDCC 3451 and its potential features provide a promising functional and safe probiotic strain for the human consumption.

中文翻译：

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从婴儿粪便中分离的干酪乳杆菌 IDCC 3451 的基因组特征和益生菌潜力

益生菌对宿主的健康益处起着重要作用。然而，它们也具有传染性或原位产生毒素的潜力；因此，需要对其安全性进行全面评估。在这项研究中，我们报告了从婴儿粪便中新分离出的干酪乳杆菌 IDCC 3451 的基因组特征。基于酶活性和碳水化合物发酵曲线的表型分析代表了菌株的代谢特征。对鼠类小鼠模型的抗菌素耐药性、生物胺产生和细胞毒性的安全评估表明其作为益生菌菌株的安全使用。我们对干酪乳杆菌 IDCC 3451 的遗传背景及其潜在特征的发现为人类消费提供了一种有前途的功能性和安全的益生菌菌株。