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Dynamics of cercospora leaf spot disease determined by aerial spore dispersal in artificially inoculated sugar beet fields
Plant Pathology ( IF 2.7 ) Pub Date : 2021-01-09 , DOI: 10.1111/ppa.13337
Frederike Imbusch 1 , Sebastian Liebe 1 , Tobias Erven 2 , Mark Varrelmann 1
Affiliation  

Cercospora beticola is one of the most important fungal pathogens of sugar beet, causing cercospora leaf spot (CLS) disease. Due to the decreasing efficacy of various fungicides caused by resistance traits, the development of a sustainable disease management strategy has become more important. Therefore, detailed knowledge about the epidemiology of the pathogen is crucial. Until now, little was known about the spatiotemporal dispersal of C. beticola spores from the primary inoculum source. Rapid detection of C. beticola spores could facilitate a more precise and targeted disease control. Therefore, a TaqMan real‐time PCR assay for detection and quantification of C. beticola spores caught with Rotorod spore traps was established. In 2016 and 2017, field trials were conducted to monitor C. beticola aerial spore dispersal and disease development within an inoculated field and in the adjacent noninoculated area. With the established detection method, C. beticola spores were successfully quantified and used as a measure for aerial spore dispersal intensity. The analysis of the spatiotemporal spread of C. beticola spores revealed a delay and decrease of aerial spore dispersal with increasing distance from the inoculated area. Consequently, disease incidence and severity were reduced in a similar manner. These results imply that spore dispersal occurs mainly on a small scale within a field, although long distances can be overcome by C. beticola spores. Moreover, secondary aerial spore dispersal from sporulating leaf spots seems to be the main driver for CLS disease development. These results provide an important basis for further improvement of CLS control strategies.

中文翻译:

人工接种甜菜田中气生孢子扩散确定尾孢叶斑病的动态

锥尾孢Cercospora beticola)是甜菜最重要的真菌病原体之一,可引起尾孢斑病(CLS)。由于抗药性引起的各种杀菌剂功效的下降,制定可持续的疾病管理策略变得越来越重要。因此,有关病原体流行病学的详细知识至关重要。到现在为止,关于C的时空扩散还知之甚少。来自主要接种源的贝蒂科拉孢子。快速检测Ç麻疯树孢子可以促进更精确,更有针对性的疾病控制。因此,TaqMan实时PCR测定法的检测和定量Ç贝蒂科拉建立了用Rotorod孢子阱捕获的孢子。在2016年和2017年,进行了田间试验以监测Ç贝蒂科拉气生孢子的扩散和在接种区域和附近非接种区域的疾病发展。既定检测方法,Ç贝蒂科拉孢子已成功量化,并用作测量气生孢子扩散强度的方法。C的时空传播分析。贝蒂科拉孢子显示出空气孢子散布的延迟和减少,其与接种区域的距离增加。因此,以类似的方式降低了疾病的发生率和严重性。这些结果暗示孢子散布主要发生在田间小范围内,尽管长距离可以用C克服。贝蒂科拉孢子。此外,来自孢子叶斑的次级气生孢子散播似乎是CLS疾病发展的主要驱动力。这些结果为进一步改善CLS控制策略提供了重要依据。
更新日期:2021-01-09
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