A novel monolithic column incorporated with Schiff base network-1 (SNW-1) was prepared for in-tube solid phase microextraction (SPME) of antiepileptic drugs. The SNW-1 nanoparticles was synthesized by a solvent thermal method and embedded in the poly(glycidyl methacrylate-ethylene glycol dimethacrylate) (GMA-EGDMA) monolithic column by in situ polymerization. The amount of SNW-1 in polymerization solution for preparation of monolithic column was optimized. Fourier transform infrared spectrometry and scanning electron microscope were used to characterize the obtained monolithic column. The SNW-1-monolithic column was connected to the high-performance liquid chromatography (HPLC) system and applied for online extraction and analysis of antiepileptic drugs. The extraction conditions including sample solution flow rate, desorption time, acetonitrile content, pH of sample solution and sample volume were investigated. Under optimal conditions, the proposed SPME-HPLC method showed high extraction efficiency, good linearity, low limits of detection (0.2 ng/mL) and good repeatability (relative standard deviations < 2.41%). This method was also applied to the analysis of antiepileptic drugs in human plasma samples and the recoveries were in the range of 88.6–106.1% with RSDs less than 3.51%.

制备了与席夫碱网络-1（SNW-1）结合的新型整体柱，用于抗癫痫药的管内固相微萃取（SPME）。SNW-1纳米粒子通过溶剂热法合成，并通过原位聚合法包埋在聚甲基丙烯酸缩水甘油酯-乙二醇二甲基丙烯酸酯（GMA-EGDMA）整体柱中。优化了用于制备整体柱的聚合溶液中SNW-1的量。用傅里叶变换红外光谱和扫描电子显微镜对所得整体柱进行表征。SNW-1整体柱连接到高效液相色谱（HPLC）系统，并用于抗癫痫药的在线提取和分析。提取条件包括样品溶液流速，解吸时间，研究了乙腈含量，样品溶液的pH和样品体积。在最佳条件下，建议的SPME-HPLC方法显示出高提取效率，良好的线性，低检测限（0.2 ng / mL）和良好的重复性（相对标准偏差<2.41％）。该方法还用于分析人血浆样品中的抗癫痫药，回收率在88.6-106.1％之间，RSD小于3.51％。