Dickkopf1 (DKK1) is a secreted inhibitor for the Wnt signalling, which is involved in cell proliferation, tissue regeneration and embryonic development. Using CRISPR/Cas9 editing, we established a homozygous mutant DKK1 human embryonic stem cell line (WAe001-A-21). It has a 41 bp deletion in exon 2 of DKK1, leading to its coding frame shift. The WAe001-A-21 cell line maintains a normal karyotype, pluripotency markers, typical stem cell morphology and the ability to differentiate into three germ layers.

中文翻译：

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使用CRISPR / Cas9产生DKK1纯合敲除人胚胎干细胞系WAe001-A-21

Dickkopf1（DKK1）是Wnt信号的分泌抑制剂，参与细胞增殖，组织再生和胚胎发育。使用CRISPR / Cas9编辑，我们建立了纯合突变体DKK1人胚胎干细胞系（WAe001-A-21）。它在DKK1的外显子2中缺失了41 bp，导致其编码移码。WAe001-A-21细胞系保持正常的核型，多能性标记，典型的干细胞形态以及分化为三个胚层的能力。