Listeria monocytogenes (L. monocytogenes) is one of the top five dangerous foodborne pathogens which widely exists in most raw food and has approximately 30 % mortality rate in high-risk groups. Food safety caused by foodborne pathogens is still a major problem faced by humans in all world. The conventional analytical methods currently used involve complex bacteriological tests and usually take several days for incubation and analysis. Thus, in order to prevent the spread of disease, the development of a detection method with high speed, high accuracy and sensitivity is urgent and necessary. Herein, we developed an approach for the identification and magnetic capture of L. monocytogenes by using core@shell Fe₃O₄@silica nanoparticles terminated with hydroxyl or amine groups. Our results show that both amine- and hydroxyl-terminated Fe₃O₄@silica core@shell nanoparticles functionalized with specific antibodies, present 95.2 %±6.2 % and 98.6 %±0.3 % capture efficacies, respectively. However, without conjugating the specific antibodies, the hydroxyl-terminated Fe₃O₄@silica nanoparticles exhibit 17.6 %±1.6 % efficacy, while the amine-terminated one remains 93.2 %±9.2 % capture efficiency ascribed to the high affinity. This study quantitatively uncovers the specific and non-specific recognitions relevant to the molecular-scale physiochemical interactions between the microorganisms and the functionalized particles, and the results from this work can be generalized and extended to other bacterial species by changing antibodies, also have important implications in developing advanced analytic methods.

单核细胞增生李斯特氏菌（L.菌等）是广泛存在于大多数生食，拥有约死亡率在高危人群中30％的五大危险的食源性致病菌之一。由食源性病原体引起的食品安全仍然是全世界人类面临的主要问题。当前使用的常规分析方法涉及复杂的细菌学测试，通常需要几天的时间进行孵育和分析。因此，为了防止疾病的传播，迫切且有必要开发一种高速，高精度和灵敏的检测方法。在此，我们开发了用于鉴定和磁性捕获的方法L.单核细胞通过使用芯壳@的Fe ₃ ö ₄二氧化硅纳米颗粒被羟基或胺基封端。我们的结果表明，以胺和羟基封端的Fe ₃ O ₄硅核@壳纳米粒子均被特异性抗体功能化，分别具有95.2％±6.2％和98.6％±0.3％的捕获效率。然而，在不结合特异性抗体的情况下，羟基封端的Fe ₃ O ₄二氧化硅纳米粒子表现出17.6％±1.6％的功效，而被胺封端的纳米粒子仍具有93.2％±9.2％的捕获效率，这归因于高亲和力。这项研究定量地发现了与微生物和功能化颗粒之间的分子尺度生理化学相互作用有关的特异性和非特异性识别，并且这项工作的结果可以通过改变抗体而推广到其他细菌物种，也具有重要意义。开发先进的分析方法。