To efficiently remove nitrogen and phosphorus from secondary effluent with low values of COD/TN, a novel biological aerated filter (BAF) utilizing calcined pyrite with a large specific surface area (SSA) and pore diameter (PD) was designed to address this challenge. From the perspective of nutrients removal performance, and the corresponding effluent total nitrogen (TN) and PO₄^3--P in the calcined pyrite autotrophic denitrification (CPAD) process decreased from 40.21 to 1.07 mg/L to 1.22 and 0.14 mg/L, respectively. Furthermore, the nutrients removal kinetics analysis showed that the CPAD and pyrite autotrophic denitrification (PAD) processes could be fitted with Half-order and Zero-order reactions via kinetics analysis, respectively, indicating that the TN removal performance of CPAD processes was better than that of the PAD process. Moreover, CPAD combined with sulfur autotrophic denitrification (SAD) processes was fitted by First-order reaction, and the TN removal performance was further enhanced over the CPAD process. From the perspective of microregulation, Fe²⁺ production in the PAD and CPAD processes could accelerate the electron transfer rate by increasing electron transport system activity (ETSA) and reducing electrochemical impedance spectroscopy (EIS). Moreover, Fe²⁺ stimulated microbes to produce more proteins (PN) and C₁₀-HSL, which improved biofilm stability and interspecific communication processes. Notably, nitrifiers and autotrophic denitrifiers were simultaneously enriched via detection of high-throughput sequencing of 16 S rRNA genes, which verified the feasibility of simultaneous nitrification and autotrophic denitrification. Therefore, BAF with calcined pyrite and sulfur as composite fillers have a considerable advantage in nutrients removal.

为了有效地以低COD / TN值从次级废水中去除氮和磷，设计了一种新型的生物曝气滤池（BAF），利用具有较大比表面积（SSA）和孔径（PD）的煅烧黄铁矿来应对这一挑战。从营养去除性能的角度来看，煅烧黄铁矿自养反硝化（CPAD）过程中相应的废水总氮（TN）和PO ₄ ^3- -P从40.21降至1.07 mg / L降至1.22和0.14 mg / L，分别。此外，营养物去除动力学分析表明，CPAD和黄铁矿自养反硝化（PAD）工艺可以适合半阶和零阶分别通过动力学分析反应，表明CPAD工艺的TN去除性能优于PAD工艺。此外，CPAD与硫自养反硝化（SAD）工艺相结合通过一级反应进行装配，并且与CPAD工艺相比，TN去除性能得到了进一步提高。从微调节的角度来看，PAD和CPAD工艺中产生的Fe ²⁺可通过增加电子传输系统活性（ETSA）和降低电化学阻抗谱（EIS）来加快电子传输速率。此外，Fe ²⁺刺激的微生物产生更多的蛋白质（PN）和C ₁₀-HSL，可改善生物膜的稳定性和种间通讯过程。值得注意的是，通过检测16 S rRNA基因的高通量测序，可以同时富集硝化剂和自养反硝化剂，这证明了同时硝化和自养反硝化的可行性。因此，以煅烧的黄铁矿和硫作为复合填料的BAF在去除营养物方面具有相当大的优势。