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Efficacy assessment of entomopathogenic nematodes native to Morocco against the white grubs Rhizotrogus obesus Lucas and Geotrogus olcesii Fairmaire (Coleoptera: Scarabaeidae)
Crop Protection ( IF 2.8 ) Pub Date : 2021-01-09 , DOI: 10.1016/j.cropro.2021.105534
Youssef Benseddik , Abdelmalek Boutaleb Joutei , Malaynine Laghfiri , Abdelali Blenzar , Said Amiri , Said Ezrari , Azzam Saleh , Fouad Mokrini , Abdessalem Tahiri , Abdelfettah A. Dababat , Rachid Lahlali

The white grubs Rhizotrogus obesus Lucas and Geotrogus olcesii Fairmaire (Coleoptera: Scarabaeidae) are two of the most problematic soil-dwelling pests of some annual crops in Morocco, where they may become a target for entomopathogenic nematodes (EPN). In this study, fourteen EPN strains reported from different fields in Morocco were investigated for their efficacy against both scarab species, under laboratory and greenhouse conditions. In laboratory experiments, seven strains of Heterorhabditis bacteriophora (HB-MOR2, HB-MOR6, HB-MOR1, HB-MOR5, HB-MOR7, HB-MOR4, and HB-MOR8) and one strain of Steinernema feltiae (SF-MOR10), provided remarkable efficacy against R. obesus (38–73% of mortalities). The EPN strains of Steinernema sp.2 (Sfg-MOR12), H. bacteriophora (HB-MOR4), Steinernema sp.1 (Sfg-MOR11), and H. bacteriophora (HB-MOR1) caused mortality rates ranging from 53 to 68% against G. olcesii. In greenhouse experiments where chlorpyrifos-ethyl was used as a positive control, larval mortality of R. obesus caused by both strains of H. bacteriophora, HB-MOR6 (81%) and HB-MOR2 (75%), was higher than that obtained by chlorpyrifos-ethyl (73%). The mortality rate in G. olcesii caused by Steinernema sp.2 (Sfg-MOR12) (77%) was the same as for chlorpyrifos-ethyl treatment (74%). Therefore, it can be concluded that EPN can constitute a promising biological alternative for the sustainable control of R. obesus and G. olcesii.



中文翻译:

摩洛哥原产的昆虫病原线虫对白obeRhozotrogus obesus Lucas和Geotrogus olcesii Fairmaire(鞘翅目:Scarabaeidae)的功效评估

gRhozotrogus obesus Lucas和Geotrogus olcesii Fairmaire(鞘翅目:Scarabaeidae)是摩洛哥一些一年生作物中最成问题的土壤居住害虫中的两种,它们可能成为昆虫病原线虫(EPN)的目标。在这项研究中,调查了摩洛哥不同领域报道的14种EPN菌株在实验室和温室条件下对两种圣甲虫的功效。在实验室实验中,发现了7株细菌性异型杆菌细菌(HB-MOR2,HB-MOR6,HB-MOR1,HB-MOR5,HB-MOR7,HB-MOR4和HB-MOR8)和一株Steinernema菌丝体(SF-MOR10)。对R. obesus提供了显着的功效(死亡率的38-73%)。的EPN菌株SP.2(SFG-MOR12),嗜菌(HB-MOR4),斯氏SP.1(SFG-MOR11),和嗜菌(HB-MOR1)引起的死亡率范围从53至68相对于油酵母菌的%。在温室实验中毒死蜱乙基用作阳性对照的幼虫死亡率R.眼金枪鱼引起的两种菌株嗜菌, HB-MOR6(81%)和HB-MOR2(75%),比获得了更高的由毒死rif-乙基(73%)。Steinernema引起的油青霉的死亡率sp.2(Sfg-MOR12)(77%)与毒死rif乙基处理的相同(74%)。因此,可以得出结论,EPN可以构成为可持续控制一个有前途的生物替代R.眼金枪鱼G. olcesii

更新日期:2021-01-14
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