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Generation-specific and interactive effects of pesticide and antidepressant exposure in a fish model call for multi-stressor and multigenerational testing
Aquatic Toxicology ( IF 4.5 ) Pub Date : 2021-01-08 , DOI: 10.1016/j.aquatox.2021.105743
Eli S.J. Thoré , Floor Van Hooreweghe , Charlotte Philippe , Luc Brendonck , Tom Pinceel

Ecological risks of a pollutant are typically assessed via short-term exposure of model organisms to that single compound. Such tests are informative, but cannot ascertain effects of long-term and multigenerational mixed-stressor exposure with which organisms are often confronted in their natural environment. Therefore, full life-cycle and multigenerational tests are needed. Yet, these are hampered due to long lifespans and generation times of many standard laboratory species, in particular for vertebrates such as fish. With a typical lifespan of 6 months and a generation time of about 3 months, the turquoise killifish (Nothobranchius furzeri) may be an ideal model for multigenerational testing. In this study, we assessed the impact of full life-cycle exposure to the emerging pollutant fluoxetine (0, 0.5 μg/L) in combination with chronic exposure during adulthood to the pesticide 3,4-dichloroaniline (0, 50, 100 μg/L) over two successive generations of N. furzeri. Overall, both life-history and behaviour were affected by exposure to fluoxetine and 3,4-DCA. Inhibitory effects of single chemical exposure on growth and fecundity were generation-dependent, while enhanced swimming acceleration and feeding in response to fluoxetine were dependent on the presence of 3,4-DCA. Together, these findings show the relevance of a multi-stressor approach across successive generations. Although full life-cycle and multigenerational tests are typically assumed to be impractical and costly for fish, we deliver an effective demonstration that such studies are possible within a timespan of less than 6 months with the killifish N. furzeri as a model organism.



中文翻译:

鱼模型中农药和抗抑郁剂暴露的特定世代和交互作用要求进行多压力和多代测试

污染物的生态风险通常通过模型生物短期接触该单一化合物来评估。这种测试是有益的,但不能确定长期和多代混合应激源暴露的影响,而有机物通常在自然环境中面临这种影响。因此,需要完整的生命周期和多代测试。然而,由于许多标准实验室物种的寿命长和产生时间长,这些问题受到阻碍,特别是对于脊椎动物(例如鱼类)而言。绿松石kill鱼Nothobranchius furzeri)的典型寿命为6个月,生成时间约为3个月。)可能是多代测试的理想模型。在这项研究中,我们评估了整个生命周期接触新兴污染物氟西汀(0,0.5μg/ L)以及成年后长期接触农药3,4-二氯苯胺(0,50,100μg/ L)连续两个世代的N. furzeri。总体而言,接触氟西汀和3,4-DCA会影响生活史和行为。单一化学物质暴露对生长和繁殖力的抑制作用取决于世代,而增加的游泳加速和对氟西汀的摄食依赖于3,4-DCA的存在。总之,这些发现表明了在多代人之间采用多压力方法的重要性。尽管通常认为完整的生命周期测试和多代测试对于鱼来说是不切实际且昂贵的,但我们提供了有效的证明,即以杀死小鱼N. furzeri为模型生物,可以在不到6个月的时间内进行此类研究。

更新日期:2021-01-16
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