Anesthesia is a stress mitigation technique widely used during transportation and short-term human manipulation, such as labeling and measurement of body parameters of aquatic animals. This is because anesthetics affect the content of neurotransmitters, exerting various effects on the body. This study determined optimal anesthetic concentration for cuttlefish by measuring the anesthetic and recovery duration of Sepia pharaonis. This was assessed with Nuclear magnetic resonance (NMR) combined with high performance liquid chromatography (HPLC) detection of nervous system metabolites and specific changes in the content of six neurotransmitters. Successful anesthetization of cuttlefish was observed with ethanol concentration (10–30 mL L⁻¹). The cuttlefish recovered after anesthesia, with the longest exposure time being 60 min at a concentration of 10 mL L⁻¹ ethanol. Likewise, the cuttlefish also reached a sedative state at a concentration below 5 mL L⁻¹ ethanol, but with a maximum exposure time of 270 min. We concluded that the action of ethanol attributed to the 10–20% increase of glycine and tyrosine using NMR and HPLC analysis. We postulate the anesthetic role of ethanol in cuttlefish led to increased inhibitory neurotransmitters gamma-Aminobutyric acid, (GABA) and Glycine (Gly) while it reduced the levels of excitatory neurotransmitters acetylcholine (Ach), dopamine (DA) and 5-hydroxytryptamine (5-HT).However, cuttlefish recovery from anesthesia was probably due to rising Ach and Glutamate (Glu) levels.

麻醉是一种缓解压力的技术，广泛用于运输和短期人为操作（例如标记和测量水生动物的身体参数）期间。这是因为麻醉剂会影响神经递质的含量，从而对人体产生各种影响。本研究通过测量法老乌贼的麻醉和恢复时间确定了乌贼的最佳麻醉浓度。通过核磁共振（NMR）结合高效液相色谱（HPLC）检测神经系统代谢产物和六种神经递质含量的特定变化进行评估。在乙醇浓度为10–30 mL L ^{-1的情况下}，乌贼成功麻醉）。乌贼在麻醉后恢复，在10 mL L ^-1乙醇浓度下的最长暴露时间为60分钟。同样，乌贼在低于5 mL L ^-1乙醇的浓度下也达到镇静状态，但最大暴露时间为270分钟。我们得出的结论是，使用NMR和HPLC分析，乙醇的作用归因于甘氨酸和酪氨酸增加了10–20％。我们推测乙醇在乌贼中的麻醉作用会导致抑制性神经递质γ-氨基丁酸（GABA）和甘氨酸（Gly）的增加，而乙醇会降低兴奋性神经递质的乙酰胆碱（Ach），多巴胺（DA）和5-羟色胺（5 -HT）。但是，从麻醉中恢复乌贼可能是由于Ach和谷氨酸（Glu）水平升高。