Probiotic supplementation in fish feed is becoming popular as a viable alternative to curb the indiscriminate use of antibiotics in aquaculture. The effect of inactivated Lactobacillus plantarum on growth performance and health related indices of black sea bream (Acanthopagrus schlegelii) fingerlings was studied, using different concentrations of a commercial inactivated L. plantarum product (Immuno-LP20): D1 (control, 0 mg/kg), D2 (100 mg/kg), D3 (200 mg/kg), D4 (400 mg/kg), D5 (800 mg/kg), and D6 (1600 mg/kg). After an eight-week feeding trial during which the diets were fed to triplicate groups of fish (initial weight: 1.58 ± 0.05 g), D2, D3, and D4 produced fish with significantly higher growth parameters than the control fish (P < 0.05), whilst all fish fed the diets with L. plantarum had significantly improved feed utilization parameters than the control group. Serum total protein content, and lysozyme activity significantly increased in fish fed D4 than the control fish, whilst complement 4 content was higher in fish fed almost all the inactivated probiotic diets (except D5) than the control fish. Serum superoxide dismutase activity was significantly higher in fish fed all the Immuno-LP20 diets except D2, and liver glutathione peroxidase activity in D2, D3, and D4 fish were significantly higher than in the control fish. Intestinal structure was also significantly improved in fish fed the Immuno-LP20 diets. According to these results, using inactivated L. plantarum as dietary supplement could be beneficial in the production of black sea bream at an optimum inclusion rate similar to 100 mg/kg ≤ 400 mg/kg Immuno-LP20.

鱼饲料中添加益生菌作为抑制水产养殖中滥用抗生素的可行替代方法正变得越来越普遍。灭活的影响植物乳杆菌生长性能和黑鲷（与健康相关的指标Acanthopagrus许氏）鱼种进行了研究，利用不同浓度的商业灭活的大号。植物乳品（Immuno-LP20）：D1（对照，0 mg / kg），D2（100 mg / kg），D3（200 mg / kg），D4（400 mg / kg），D5（800 mg / kg），和D6（1600 mg / kg）。经过为期八周的饲喂试验，在该试验中，将饲料饲喂三组鱼（初始重量：1.58±0.05克），D2，D3和D4生产的鱼的生长参数明显高于对照鱼（P  <0.05），而所有鱼类均以L饲喂日粮。车前草的饲料利用率参数比对照组明显提高。饲喂D4的鱼的血清总蛋白含量和溶菌酶活性显着高于对照鱼，而饲喂几乎所有灭活益生菌饲料（D5除外）的鱼的补体4含量均高于对照鱼。除D2外，所有Immuno-LP20日粮饲喂的鱼的血清超氧化物歧化酶活性均显着较高，而D2，D3和D4鱼的肝谷胱甘肽过氧化物酶活性则显着高于对照鱼。饲喂Immuno-LP20日粮的鱼的肠结构也显着改善。根据这些结果，使用灭活的大号。植物性膳食补充剂可能对黑鲷的生产有益，最佳包裹率类似于100 mg / kg≤400 mg / kg Immuno-LP20。