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Involvement of salicylic acid in cold priming-induced freezing tolerance in wheat plants
Plant Growth Regulation ( IF 4.2 ) Pub Date : 2020-10-05 , DOI: 10.1007/s10725-020-00671-8
Weiling Wang , Xiao Wang , Xinyu Zhang , Yao Wang , Zhongyang Huo , Mei Huang , Jian Cai , Qin Zhou , Dong Jiang

Freezing is one of the most damaging abiotic stress factors. Cold priming has been shown to enhance freezing tolerance in wheat. However, the underlying mechanisms are unclear. Salicylic acid (SA) is an important signal molecule involved in plant responses to abiotic and biotic stresses. This study aims to investigate the role of SA in cold priming-induced freezing tolerance in wheat plants. The results showed that the expression of gene encoding phenylalanine ammonia-lyase (PAL, a key enzyme involved in SA biosynthesis) and the level of endogenous SA (both free and conjugated) were significantly up-regulated following cold priming treatment. The role of SA in cold priming-induced freezing tolerance was further explored using the donor and inhibitor of endogenous SA. l -α-aminooxy-β-phenylpropionic acid (AOPP, a specific inhibitor of PAL) pretreatment reversed the increment of SA level induced by cold priming treatment, attenuated the positive effects of cold priming on antioxidant capacity (as indicated by the contents of MDA, ASA and GSH, and the activities of APX, GR, DHAR and MDHAR) and cold-responsive genes expression (such as AOX1a , IRI2 and HSP70 ) under the later freezing stress as well as the freezing tolerance (as indicated by the photochemical activity, electrolyte leakage and biomass). The application of exogenous SA could obviously counteract the negative effects of AOPP. Findings of the present study suggested that the roles of SA in cold priming-induced freezing tolerance in wheat might involve regulation of antioxidant capacity and cold-responsive gene expression.

中文翻译:

水杨酸对小麦冷引发抗冻性的影响

冷冻是最具破坏性的非生物胁迫因素之一。冷启动已被证明可以提高小麦的抗冻性。然而,潜在的机制尚不清楚。水杨酸 (SA) 是一种重要的信号分子,参与植物对非生物和生物胁迫的反应。本研究旨在研究 SA 在小麦植株冷引发诱导的抗冻性中的作用。结果表明,冷引发处理后,苯丙氨酸解氨酶(PAL,参与SA生物合成的关键酶)基因的表达和内源性SA(游离和结合)的水平显着上调。使用内源性 SA 的供体和抑制剂进一步探讨了 SA 在冷启动诱导的冷冻耐受中的作用。l -α-氨基氧基-β-苯基丙酸(AOPP,PAL 的特异性抑制剂)预处理逆转了冷启动处理诱导的 SA 水平增加,减弱了冷启动对抗氧化能力的积极影响(如 MDA、ASA 和 GSH 的含量以及 APX、GR、 DHAR 和 MDHAR)和冷响应基因的表达(如 AOX1a、IRI2 和 HSP70)在后期冷冻胁迫下以及耐寒性(如光化学活性、电解质泄漏和生物量所示)。外源性SA的应用可以明显抵消AOPP的负面影响。本研究结果表明,SA 在小麦冷启动诱导的抗冻性中的作用可能涉及抗氧化能力和冷响应基因表达的调节。减弱冷启动对抗氧化能力(如 MDA、ASA 和 GSH 的含量,以及 APX、GR、DHAR 和 MDHAR 的活性)和冷响应基因表达(如 AOX1a、IRI2 和 HSP70)的积极影响在后期的冷冻胁迫下以及耐冷冻性(如光化学活性、电解质泄漏和生物量所示)。外源性SA的应用可以明显抵消AOPP的负面影响。本研究结果表明,SA 在小麦冷启动诱导的抗冻性中的作用可能涉及抗氧化能力和冷响应基因表达的调节。减弱冷启动对抗氧化能力(如 MDA、ASA 和 GSH 的含量,以及 APX、GR、DHAR 和 MDHAR 的活性)和冷响应基因表达(如 AOX1a、IRI2 和 HSP70)的积极影响在后期的冷冻胁迫下以及耐冷冻性(如光化学活性、电解质泄漏和生物量所示)。外源性SA的应用可以明显抵消AOPP的负面影响。本研究结果表明,SA 在小麦冷启动诱导的抗冻性中的作用可能涉及抗氧化能力和冷响应基因表达的调节。DHAR 和 MDHAR)和冷响应基因(如 AOX1a、IRI2 和 HSP70)在后期冷冻胁迫下的表达以及耐寒性(如光化学活性、电解质泄漏和生物量所示)。外源性SA的应用可以明显抵消AOPP的负面影响。本研究结果表明,SA 在小麦冷启动诱导的抗冻性中的作用可能涉及抗氧化能力和冷响应基因表达的调节。DHAR 和 MDHAR)和冷响应基因的表达(如 AOX1a、IRI2 和 HSP70)在后期冷冻胁迫下以及耐寒性(如光化学活性、电解质泄漏和生物量所示)。外源性SA的应用可以明显抵消AOPP的负面影响。本研究结果表明,SA 在小麦冷启动诱导的抗冻性中的作用可能涉及抗氧化能力和冷响应基因表达的调节。
更新日期:2020-10-05
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