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Base editing in rice: current progress, advances, limitations, and future perspectives
Plant Cell Reports ( IF 6.2 ) Pub Date : 2021-01-10 , DOI: 10.1007/s00299-020-02656-3
Rajesh Yarra 1 , Lingaraj Sahoo 2
Affiliation  

Key message

Base editing is one of the promising genome editing tools for generating single-nucleotide changes in rice genome.

Abstract

Rice (Oryza sativa L.) is an important staple food crop, feeding half of the population around the globe. Developing new rice varieties with desirable agronomic traits is necessary for sustaining global food security. The use of genome editing technologies for developing rice varieties is pre-requisite in the present scenario. Among the genome editing technologies developed for rice crop improvement, base editing technology has emerged as an efficient and reliable tool for precise genome editing in rice plants. Base editing technology utilizes either adenosine or cytidine base editor for precise editing at the target region. A base editor (adenosine or cytidine) is a fusion of catalytically inactive CRISPR/Cas9 domain and adenosine or cytidine deaminase domain. In this review, authors have discussed the different adenine and cytosine base editors developed so far for precise genome editing of rice via base editing technology. We address the current progress, advances, limitations, as well as future perspectives of the base editing technology for rice crop improvement.



中文翻译:

水稻碱基编辑:当前进展、进展、局限和未来展望

关键信息

碱基编辑是用于在水稻基因组中产生单核苷酸变化的有前途的基因组编辑工具之一。

抽象的

水稻(Oryza sativaL.) 是一种重要的主食作物,为全球一半的人口提供食物。开发具有理想农艺性状的新水稻品种对于维持全球粮食安全是必要的。在当前情况下,使用基因组编辑技术开发水稻品种是先决条件。在为改良水稻作物而开发的基因组编辑技术中,碱基编辑技术已成为一种高效可靠的水稻基因组精确编辑工具。碱基编辑技术利用腺苷或胞苷碱基编辑器在目标区域进行精确编辑。碱基编辑器(腺苷或胞苷)是无催化活性的 CRISPR/Cas9 结构域和腺苷或胞苷脱氨酶结构域的融合体。在本次审查中,作者讨论了迄今为止为通过碱基编辑技术对水稻进行精确基因组编辑而开发的不同腺嘌呤和胞嘧啶碱基编辑器。我们讨论了水稻作物改良碱基编辑技术的当前进展、进步、局限性以及未来前景。

更新日期:2021-01-10
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