The aims of the present study were to evaluate the effects of different ventilation systems and sucrose concentrations on the growth, photosynthetic pigment content, and chemical composition of Mentha arvensis L. and M. viridis L. Photon flux (PPF) and growth were also evaluated based on M. arvensis heterotrophic, photomixotrophic, and photoautotrophic culture systems. Nodal segment explants were cultured on medium with 0, 15, and 30 g L⁻¹ sucrose and four alternative membrane systems (AMS) in culture medium, namely no membrane (NMS), one (AMS1), two (AMS2), and four (AMS4) membranes. Mentha arvensis cultivation on medium containing 15 g L⁻¹ of sucrose and four membrane (AMS4) led to the highest dry weight accumulation. Mentha viridis reached the same result with medium containing 30 g L⁻¹ sucrose and AMS2 and AMS4. Mentha arvensis produced higher chlorophyll a, b and total chlorophyll, as well as carotenoids when cultured on medium with 30 g L⁻¹ of sucrose and AMS4 and M. viridis when cultured on medium with 15 g L⁻¹ of sucrose and AMS4. The NMS without sucrose favored higher pulegone and AMS4 induced higher menthol, menthone, and limonene from M. arvensis cultured in vitro. Furthermore, AMS2 induced menthol production and AMS1 increased trans-sabinene, linalol, and limonene content in M. viridis. Mentha arvensis plantlets cultivated under photoautotrophic system with 130 μmol m⁻² s⁻¹ showed improved growth and the highest leaf area. Thus, the recommendation to increase upper biomass and plantlet quality in mint species lies on employing an alternative membrane system under higher PPF.

本研究的目的是评估不同的通风系统和蔗糖浓度对薄荷，薄荷和芒草的生长，光合色素含量和化学组成的影响。还评估了光子通量（PPF）和生长基于M. arvensis异养，光合营养和光自养培养系统。将节段外植体在含有0、15和30 g L ^-1蔗糖的培养基以及四种可选膜系统（AMS）的培养基中培养，即无膜（NMS），一种（AMS1，），两种（AMS2）和四种（AMS4）膜。在15 g L ^-1的培养基上栽培薄荷蔗糖和四层膜（AMS4）的干重最大。薄荷菜用含有30 g L ^-1蔗糖和AMS2和AMS4的培养基达到了相同的结果。当在含30 g L ^-1蔗糖和AMS4的培养基上培养时，薄荷脑产生更高的叶绿素a，b和总叶绿素，以及类胡萝卜素。当在含15 g L ^-1蔗糖和AMS4的培养基上培养时，薄荷。没有蔗糖的NMS有利于较高的pulegone，而AMS4可以诱导体外培养的欧文分支杆菌较高的薄荷醇，薄荷酮和柠檬烯。。此外，AMS2诱导了薄荷醇的产生，而AMS1则增加了M. viridis中反式-烯，芳樟醇和li烯的含量。在130μmolm ^-2  s ^-1的光合自养系统下栽培的薄荷脑幼苗生长得到改善，叶面积最大。因此，提高薄荷物种上部生物量和幼苗质量的建议是在PPF较高的情况下采用替代膜系统。