Shoot regeneration from pluripotent cell masses is an important process for genetic improvement of Anthurium andraeanum through Agrobacterium-mediated transformation. However, the mechanisms underlying pluripotent cell induction are not well understood. Here, three half-leaf explant samples collected after 0-, 2-, and 30-d culture on callus-inducing medium were used for RNA isolation and transcriptome profiling. In total, 225,752 non-redundant unigenes were assembled with a mean length of 1299 bp. Differentially expressed genes among the three samples were identified by comparative transcriptome analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed the greatest number of differentially expressed genes in the comparison between 0- and 2-d cultures were related to the “ribosome.” Many differentially expressed genes in the comparison between 2- and 30-d cultures were involved in “plant hormone signal transduction,” “starch and sucrose metabolism,” and “phenylpropanoid biosynthesis.” Further investigation was focused on genes related to transcription factors, epigenetic regulation, nitrous oxide metabolism, and homeostasis of plant hormones auxin, cytokinin, brassinolide, and strigolactone. Experiments were designed to confirm the effects of nitrous oxide and strigolactone on callus formation in Anthurium andraeanum. Callus formation was enhanced by exogenous application of sodium nitroprusside, which produces nitrous oxide, but was inhibited under rac-GR24 (a strigolactone analogue) treatment. Our comparative transcriptome analysis revealed that the signal molecule nitrous oxide and plant hormones auxin, cytokinin, and brassinolide act as positive regulators in promoting callus formation, while strigolactone may inhibit callus formation. Transcriptional and epigenetic regulations also play important roles in pluripotent cell induction in Anthurium andraeanum.

从多能细胞团再生芽是通过农杆菌遗传改良红掌的重要过程介导的转化。但是，对多能细胞诱导的机制尚不十分了解。在这里，在愈伤组织诱导培养基上培养0、2和30天后收集的三个半叶外植体样品用于RNA分离和转录组谱分析。总共组装了225,752个非冗余单基因，平均长度为1299 bp。通过比较转录组分析鉴定了三个样品中差异表达的基因。京都基因与基因组百科全书（KEGG）分析显示，在0维和2维培养物的比较中，差异表达基因的数量最多，与“核糖体”有关。在2和30天培养的比较中，许多差异表达的基因参与了“植物激素信号转导”，“淀粉和蔗糖代谢，”和“苯丙烷生物合成”。进一步的研究集中在与转录因子，表观遗传调控，一氧化二氮代谢和植物激素生长素，细胞分裂素，油菜素内酯和松香内酯的体内稳态有关的基因上。设计实验以确认一氧化二氮和异内酯对小鼠愈伤组织形成的影响。红掌。通过外源施用硝普钠提高了愈伤组织的形成，硝普钠产生一氧化二氮，但在rac-GR24（一种内酯基内酯类似物）处理下受到抑制。我们的比较转录组分析显示，信号分子一氧化二氮和植物激素生长素，细胞分裂素和油菜素内酯在促进愈伤组织形成中起正调节剂的作用，而strigolactone可能会抑制愈伤组织的形成。转录和表观遗传调控在红掌的多能细胞诱导中也起着重要作用。