The present study investigates the effects of particle size on chemical and structural features of polyethylene glycol (PEG)-assisted 1-butyl-3-methyl imidazolium chloride ([BMIM][Cl]) pretreatment of sugarcane bagasse (SCB) by response surface methodology approach (RSM). Three particle size ranges named fine, medium, and coarse separated by mesh numbers 70, 30, and 14 were considered for this study. Temperature, time, and PEG concentration in the ranges of 100–160 °C, 60–120 min, and 1–5%(w/w), respectively, were chosen as the influencing parameters of the RSM. Percentage of glucan, xylan, and acid insoluble lignin (AIL) in the pretreated samples was evaluated as the responses. The optimization of the pretreatment process was conducted for each particle size, consequently the enzymatic hydrolysis of the samples treated in the optimum points implemented to determine the particle size range yielding the highest SCB conversion. Crystallinity index and crystallite size of the pretreated SCB were measured as the structural characterization of the lignocellulosic biomass. Glucan content of the samples pretreated in the optimum conditions was 62.8, 66.63, and 60.6% for fine, medium, and coarse particles, respectively. The results of 24 h enzymatic conversion of the SCB samples pretreated in optimum conditions were as 66.63, 76.01, and 53.77%, for fine, medium, and coarse particles, respectively. This study revealed that particle size has a significant effect on the pretreatment process, and medium particles are mostly affected by different process parameters and have had the highest enzymatic conversion.

本研究通过响应面法研究了粒径对聚乙二醇（PEG）辅助的1-丁基-3-甲基咪唑鎓氯化物（[BMIM] [Cl]）预处理甘蔗渣（SCB）的化学和结构特征的影响方法（RSM）。本研究考虑了三个粒度范围，分别是细度，中度和粗度，分别由网眼号70、30和14分隔。选择温度，时间和PEG浓度分别为100–160°C，60–120分钟和1–5％（w / w）作为RSM的影响参数。将葡聚糖，木聚糖和酸不溶性木质素（AIL）在预处理样品中的百分比作为响应。针对每种粒径进行了预处理工艺的优化，因此，在确定最佳点的最佳位置处理的样品的酶水解作用可实现产生最高SCB转化率的粒径范围。测量预处理的SCB的结晶度指数和微晶尺寸作为木质纤维素生物质的结构特征。在最佳条件下预处理的样品中，细颗粒，中颗粒和粗颗粒的葡聚糖含量分别为62.8、66.63和60.6％。在最佳条件下预处理的SCB样品的24 h酶促转化结果分别为细颗粒，中颗粒和粗颗粒，分别为66.63、76.01和53.77％。这项研究表明，粒径对预处理过程有重大影响，介质颗粒主要受不同工艺参数的影响，并且酶转化率最高。