Parasitoids use herbivore-induced plant volatiles (HIPV´s) to locate their hosts. However, little is known about variations in HIPV´s production in genetically modified maize plants that are herbicide tolerant (singular event), insect resistant (Bt plants, singular event), or both herbicide tolerant and insect resistant, like staked events. We investigated the olfactory responses of the egg parasitoid Trichogramma pretiosum (Hymenoptera: Trichogrammatidae) to HIPV´s produced in maize (Zea mays) herbicide-tolerant and insect-resistant plants or their stacked events in response to damage caused by Spodoptera frugiperda during nighttime and daytime infestations. Real-time reverse-transcription PCR was used to assess whether the presence of one or more Bacillus thuringiensis (Bt) proteins and the time of induction of HIPV’s affected the expression of genes in plants under herbivore attack. The results showed that compounds were released during both nocturnal and diurnal infestations. However, some HIPV´s were released exclusively in infestations that started during the night by non-Bt plants and they were highly attractive to parasitoids. HIPV´s produced by non-Bt plants were more attractive to parasitoids than those released by Bt plants in infestations that started during the night. However, glyphosate-tolerant maize plants were more attractive to parasitoids than isogenic plants. The expression of the analyzed genes TPS10, TPS23, LOX10, and STC1 was higher in infestations that started during the night. In this study, we discuss the possible causes of the unresponsiveness of T. pretiosum females to HIPV´s produced by Bt maize.

寄生物利用草食动物诱导的植物挥发物（HIPV´s）定位其寄主。但是，人们对转基因玉米植物中HIPV产量变化的知之甚少，这些植物具有除草剂耐受性（单事件），抗虫性（Bt植物，单一事件），或既有抗除草剂又具有昆虫抗性（如放样事件）。我们研究了蛋的嗅觉反应寄生赤眼蜂pretiosum（膜翅目：赤眼蜂科），以在玉米产生HIPV's（玉蜀黍）的除草剂耐受性和抗虫植物或它们的堆叠事件响应于伤害引起的草地夜蛾在夜间和白天的出没。实时逆转录PCR用于评估是否存在一种或多种苏云金芽孢杆菌（Bt）蛋白质和HIPV的诱导时间影响了草食动物攻击下植物中基因的表达。结果表明，化合物在夜间和昼夜侵扰期间均释放。但是，一些HIPV仅在非Bt植物在夜间开始的侵扰中释放，它们对寄生虫非常有吸引力。由非Bt植物生产的HIPV对寄生虫的吸引力比在夜间开始的Bt植物释放的那些对寄生虫的吸引力更大。但是，耐草甘膦的玉米植物比同基因植物对寄生虫更具吸引力。在夜间开始的侵扰中，分析的基因TPS10，TPS23，LOX10和STC1的表达较高。在这项研究中，我们讨论了T. pretiosum无反应的可能原因。 Bt玉米生产的HIPV的雌性。