Andrographis paniculata is traditionally used for many diseases and scientifically proven for anti-cancer property. Andrographolide which is the marker compound is believed to be the main contributor to the pharmacological activities. The poor solubility and bioavailability of this diterpenoid lactone could be overcome by nanoencapsulation. Reflux extraction, and followed by successive Soxhlet fractionation were used to obtain andrographolide rich extract from the herb. Spontaneous emulsion solvent diffusion was used to nanoencapsulate andrographolide using poly(lactic-co-glycolic acid) with 1% polyvinyl alcohol as emulsifier. Nanospheres loaded with andrographolide was found to have the particle size, 163 nm; polydispersity index, 0.26 and zeta potential, - 57.85 mV. The encapsulation efficiency and in vitro drug release were 80.0% and 84.2%, respectively. The andrographolide nanoparticles could inhibit the proliferation of cervical and neuroblastoma cells with no adverse effect on normal human skin cells. Andrographolide rich extract loaded nanoparticles could inhibit the proliferation of HeLa and SH-SY5Y cells, mainly through Bax-induced apoptosis. The result was consistent with the low expression of anti-apoptotic genes (Bcl-2 and Bcl-xL) and prognostic factor (Ki-67). The tumour size of HeLa bearing mice was significantly reduced (73%) after treated with andrographolide rich nanoparticles (10 mg/kg body weight) for a month.

中文翻译：

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富含穿心莲内酯提取物的纳米胶囊对宫颈和神经母细胞瘤癌细胞的抑制作用。

穿心莲传统上用于多种疾病，并已被科学证明具有抗癌作用。据信穿心莲内酯是标志化合物，是药理活性的主要贡献者。这种二萜类内酯的溶解性和生物利用度差，可以通过纳米封装来克服。回流提取，然后连续进行索氏提取，以从草药中获得富含穿心莲内酯的提取物。自发性乳液溶剂扩散用于穿心莲内酯纳米封装，使用聚乳酸-乙醇酸共聚物（含1％聚乙烯醇）作为乳化剂。负载穿心莲内酯的纳米球的粒径为163 nm。多分散指数为0.26，ζ电位为-57.85 mV。包封率和体外药物释放分别为80.0％和84.2％，分别。穿心莲内酯纳米颗粒可以抑制子宫颈和神经母细胞瘤细胞的增殖，而对正常人的皮肤细胞没有不利影响。富含穿心莲内酯提取物的纳米颗粒可以主要通过Bax诱导的凋亡抑制HeLa和SH-SY5Y细胞的增殖。结果与抗凋亡基因（Bcl-2和Bcl-xL）和预后因子（Ki-67）的低表达相符。用富含穿心莲内酯的纳米颗粒（10 mg / kg体重）治疗一个月后，带有HeLa的小鼠的肿瘤大小显着减少（73％）。富含穿心莲内酯提取物的纳米颗粒可以主要通过Bax诱导的凋亡抑制HeLa和SH-SY5Y细胞的增殖。结果与抗凋亡基因（Bcl-2和Bcl-xL）和预后因子（Ki-67）的低表达相符。用富含穿心莲内酯的纳米颗粒（10 mg / kg体重）治疗一个月后，带有HeLa的小鼠的肿瘤大小显着减少（73％）。富含穿心莲内酯提取物的纳米颗粒可以主要通过Bax诱导的凋亡抑制HeLa和SH-SY5Y细胞的增殖。结果与抗凋亡基因（Bcl-2和Bcl-xL）和预后因子（Ki-67）的低表达相符。用富含穿心莲内酯的纳米颗粒（10 mg / kg体重）治疗一个月后，带有HeLa的小鼠的肿瘤大小显着减少（73％）。