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Enhanced two-photon excited fluorescence by ultrafast intensity fluctuations from an optical parametric generator
Applied Physics Express ( IF 2.3 ) Pub Date : 2021-01-08 , DOI: 10.35848/1882-0786/abd674
Yujiro Eto 1, 2, 3
Affiliation  

We evaluate the enhancement of two-photon excited fluorescence from fluorescent dye by ultrafast intensity fluctuations of light generated by a nanosecond optical parametric generator (OPG). By modifying the well-established techniques for measuring the second-order coherence on ultrashort timescale, we observed the enhancement of the fluorescence from Rhodamine B dye by a factor of two due to the bunching effect of light. A nanosecond OPG can be built at a much lower cost than the ultrashort pulse used in two-photon excitation microscopes. Therefore, our work paves the way toward a low-cost and efficient nonlinear microscope.



中文翻译:

来自光学参量发生器的超快速强度波动增强了双光子激发的荧光

我们通过纳秒光学参量发生器(OPG)产生的光的超快强度波动来评估荧光染料的双光子激发荧光的增强。通过修改在超短时间尺度上测量二阶相干性的成熟技术,我们观察到由于光的聚集效应,若丹明B染料的荧光增强了两倍。与两光子激发显微镜中使用的超短脉冲相比,可以以低得多的成本制造纳秒级的OPG。因此,我们的工作为低成本,高效的非线性显微镜铺平了道路。

更新日期:2021-01-08
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