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ANTIMICROBIAL RESISTANCE AND VIRULENCE GENES PROFILING OF PROTEUS SPECIES FROM POULTRY FARMS IN LAFIA, NIGERIA
bioRxiv - Microbiology Pub Date : 2021-01-19 , DOI: 10.1101/2021.01.07.425673
Mojisola Christiana Owoseni , Odula Oyigye , Bashiru Sani , Jebes Lamin , Amara Chere

The poultry industry is important in boosting food sustainability of a population; however, the poultry environment and products are susceptible to pathogen contamination arising from poor farm hygienic conditions. This study investigated the prevalence, antimicrobial resistance and virulence profile of Proteus species from the environment and products of four selected poultry farms in Lafia, Nasarawa State. Farm samples (n =216) comprising feed (64), drinking water (64) and swabs from eggshells (88) were collected and analysed for detection of Proteus species using cultural, biochemical and microscopic techniques. Antibiotics susceptibilities of isolates were also determined, and virulence genes were confirmed using Polymerase Chain Reaction. Of the total studied samples, 34.26% (74/216) were positive for Proteus species. Proteus species were more prevalent in drinking water samples (37.84%) and feed samples (33.78%) and least prevalent in eggshells (28.38%). Proteus species (n= 74) comprised P. mirabilis 78% (58/74) and Proteus vulgaris 22% (16/74). P. mirabilis was markedly higher than P. vulgaris in all the four farms sampled. Farms A and D had the highest prevalence of Proteus species, while Farms A and C (80%), and Farm D (25) has the highest prevalence of P. mirabilis and P. vulgaris respectively. The isolates' prevalence rate within and between farms, sample type, and species was not statistically significantly different (p≥0.05) from the other farms. Isolates were 100% susceptible to Amikacin and showed the highest resistance (25.7%) to tetracycline. Molecular analysis of the virulence genes of Proteus species revealed the presence of rsbA, ureC and luxS virulent genes in all the test isolates. Data generated indicates a high level of multidrug-resistant pathogenic strains of Proteus circulating in poultry farms in Lafia, Nigeria, which potentiates a significant risk of transmission of pathogenic Proteus via the food chain.

中文翻译:

尼日利亚拉菲亚家禽农场变形杆菌属物种的抗药性和致病力基因表达。

家禽业对提高人口的粮食可持续性至关重要。然而,由于恶劣的农场卫生条件,家禽的环境和产品容易受到病原体的污染。这项研究调查了纳萨瓦瓦州拉菲亚的四个家禽养殖场的环境和产品中变形杆菌属的流行,抗药性和毒力特征。收集包括饲料(64),饮用水(64)和来自蛋壳(88)的拭子的农场样本(n = 216),并使用文化,生化和显微镜技术进行分析,以检测变形杆菌物种。还确定了分离物的抗生素敏感性,并使用聚合酶链反应确认了毒力基因。在所有研究样本中,变形杆菌属物种的阳性率为34.26%(74/216)。变形杆菌种类在饮用水样品(37.84%)和饲料样品(33.78%)中更普遍,在蛋壳中(28.38%)最不普遍。变形杆菌属物种(n = 74)包括奇异变形杆菌78%(58/74)和寻常变形杆菌22%(16/74)。在所有四个采样农场中,奇异假单胞菌显着高于寻常型假单胞菌。农场A和D的变形杆菌种类患病率最高,而农场A和C(80%)和农场D(25)的疫霉菌流行率最高。猪场内和猪场之间,样本类型和种类之间的分离株患病率与其他猪场在统计学上没有显着差异(p≥0.05)。分离株对阿米卡星敏感100%,对四环素的耐药性最高(25.7%)。对变形杆菌物种的毒力基因进行的分子分析表明,存在rsbA,所有测试分离物中的ureC和luxS毒性基因。生成的数据表明,在尼日利亚拉菲亚的家禽养殖场中传播的高水平耐多种变形杆菌病原菌,可能会引起致病性变形杆菌通过食物链传播的重大风险。
更新日期:2021-01-19
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