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Race Distribution of Pyrenophora tritici-repentis in Relation to Ploidy Level and Susceptibility of Durum and Winter Bread Wheat
Canadian Journal of Plant Pathology ( IF 2 ) Pub Date : 2021-01-08
Bohan Wei, Therese Despins, Myriam R. Fernandez, Stephen E. Strelkov, Yuefeng Ruan, Robert Graf, Reem Aboukhaddour

Abstract

Tan spot is a destructive disease of wheat caused by the fungus Pyrenophora tritici-repentis (Ptr), with most fungal isolates in Canada known to produce the necrotrophic effector (NE) ToxA. In this study, 144 Ptr isolates collected from durum, winter bread wheat, and grasses in western Canada were evaluated for race classification on a host differential set. The susceptibility of 114 durum and winter bread wheat genotypes to Ptr races 2 (R2), 3 (R3), and 5 (R5), producing the NEs ToxA, ToxB, and ToxC, respectively, was evaluated in bioassays. The host genotypes were tested for the presence of Tsn1, the ToxA-sensitivity gene, by PCR analysis. Race composition varied based on the host type and ploidy level from which the isolates were recovered. R1 (ToxA and ToxC-producer) and R2 were predominant on wheat and occurred in equal frequency on durum, while R1 occurred twice as frequently as R2 on winter bread wheat. R3 was recovered only from durum wheat, and the non-pathogenic R4 was the only race recovered from grasses. Susceptibility to R2 and R3 was more common in Canadian than in European wheat, while susceptibility to R5 was more common in durum and European wheat. Tsn1 amplified at a higher percentage in Canadian (51.2 % in winter bread, 59.0 % in durum) vs. European (20.5 %) wheat genotypes. The presence of Tsn1, however, did not result in susceptibility to ToxA-producing isolates in 35.9 % of durum genotypes, indicating that other factors are involved in the Ptr-durum interaction and that ToxA-Tsn1 may not play a significant role in tetraploid wheat.



中文翻译:

小麦和小麦面包的小麦小种的种族分布与倍性水平和易感性的关系

摘要

棕褐色斑点是由小麦黑斑病菌Ptrrenophora tritici-repentisPtr)引起的小麦破坏性疾病,在加拿大,大多数真菌分离株均会产生坏死性效应物(NE)ToxA。在这项研究中,对从加拿大西部的硬粒小麦,冬小麦和草中采集的144个Ptr分离株进行了宿主差异分类的种族分类评估。在生物测定中,评估了114种硬粒小麦和冬小麦基因型对分别产生NEs ToxA,ToxB和ToxC的Ptr族2(R2),3(R3)和5(R5)的敏感性。测试了宿主基因型中是否存在Tsn1,ToxA敏感性基因,通过PCR分析。种族组成因宿主类型和倍性水平而异,从中分离出分离物。R1(ToxA和ToxC生产者)和R2在小麦上占主导地位,在硬粒小麦上的发生频率相同,而R1在冬面包小麦上的发生频率是R2的两倍。R3仅从硬粒小麦中回收,非致病性R4是从草中回收的唯一种。加拿大人对R2和R3的敏感性比欧洲小麦更常见,而硬粒小麦和欧洲小麦对R5的敏感性更普遍。与欧洲的小麦基因型(20.5%)相比,加拿大的Tsn1扩增率更高(冬季面包为51.2%,硬质小麦为59.0%)。Tsn1的存在然而,没有造成易感性硬粒基因型的35.9%的ToxA生产菌株,这表明其他因素参与PTR -durum互动和ToxA- TSN1可能无法播放四倍体小麦显著的作用。

更新日期:2021-01-08
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