ABSTRACT

Root and rust rot are common fungal diseases that decrease both the yield and quality of ginseng, resulting in severe economic losses for the ginseng industry. In this study, we explored the bacteriostatic and greenhouse control effects that the actinomycetes strain Frankia F1, extracted from the ginseng-rhizosphere soil, has on the pathogenic fungi that cause ginseng root rot (Fusarium solani) and rust rot (Cylindrocarpon destructans). We found that F1 fermentation broth inhibited the rate of mycelia growth by 80.6% and 71.1%, as well as the rate of spore germination by 87.0% and 78.6%, in F. solani. and C. destructans, respectively. Moreover, the antagonistic mechanism of F1 crude extract damaged the cell membranes of pathogens and led to a decreased ability to reduce sugar and soluble protein. The immobilised microorganisms of germ bran and germ bran charcoal improved the physical and chemical properties and soil fertility, improved the microbial community structure, increased the relative abundance of Proteobacteria, and reduced the relative abundance of Acidobacteria. An F1 biocontrol strategy could reduce economic and agricultural losses within the ginseng industry if implemented.

摘要

根腐病是常见的真菌疾病，会降低人参的产量和质量，给人参产业造成严重的经济损失。在这项研究中，我们探索了从人参-根际土壤中提取的放线菌菌株Frankia F1对引起人参根腐烂病（Fusarium solani）和锈病腐烂（Cylindrocarpon destructans）的致病真菌的抑菌和温室控制作用。我们发现，F1发酵液在茄形茄中抑制菌丝体生长的速率分别为80.6％和71.1％，以及孢子萌发的速率分别为87.0％和78.6％。和C. destructans， 分别。此外，F1粗提物的拮抗机理破坏了病原体的细胞膜，导致还原糖和可溶性蛋白质的能力下降。固定化的麸皮和胚芽木炭微生物改善了理化性质和土壤肥力，改善了微生物群落结构，增加了变形杆菌的相对丰度，并降低了酸性细菌的相对丰度。如果实施F1生物防治策略，可以减少人参产业内的经济和农业损失。