Bombyx mori nucleopolyhedrovirus (BmNPV) is one of primary silkworm pathogens and causes a serious damage of cocoon losses every year. Recent years, many works have been done to clarify the silkworm anti‐BmNPV mechanism, and a significant progress has been made in screening and studying of genes and proteins related to BmNPV infection, but several of them lacked the proofs in vivo. In this study, to further validate the function of seven newly reported genes in vivo, including BmAtlatin‐n, Bmferritin‐heavy chain (BmFerHCH), Bmthymosin (BmTHY), Bmseroin1, Bmseroin2, Bmnuclear hormone receptors 96 (BmNHR96), and BmE3 ubiquitin‐protein ligase SINA‐like 10 (BmSINAL10), the response of them in the midgut, fat body, and hemolymph of differentially resistant strains (resistant strain YeA and susceptible strain YeB) at 48 h following BmNPV infection were analyzed. The results showed that the relative stable or upregulated expression level of BmAtlatin‐n, BmTHY, Bmseroin1, and Bmseroin2 in YeA resistant strain following BmNPV infection further indicated their antiviral role in vivo, compared with susceptible YeB strain. Moreover, the significant downregulation of BmFerHCH, BmNHR96, and BmSINAL10 in both strains following BmNPV infection revealed their role in benefiting virus infection, as well as the upregulation of BmFerHCH in YeB midgut and BmSINAL10 in YeB hemolymph. These data could be used to complementary the proofs of the function of these genes in response to BmNPV infection.

中文翻译：

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验证与家蚕核多角体病毒感染相关的几种基因在体内的作用

家蚕核多角体病毒（BmNPV）是主要的蚕病原体之一，每年都会造成严重的茧损失。近年来，为阐明家蚕抗BmNPV的机制已经进行了许多工作，在筛选和研究与BmNPV感染有关的基因和蛋白质方面已经取得了重大进展，但是其中一些缺乏体内证据。在这项研究中，为进一步验证体内七个新报道的基因的功能，包括BmAtlatin-n，Bmferritin-重链（BmFerHCH），Bmthymosin（BmTHY），Bmseroin1，Bmseroin2，Bmnuclear激素受体96（BmNHR96）和BmE3泛素蛋白连接酶SINA-like 10（BmSINAL10），分析了它们在BmNPV感染后48小时对中抗性株（YeA和易感性YeB株）中肠，脂肪体和血淋巴的反应。结果表明，与易感的YeB菌株相比，BmNPV感染后的YeA耐药菌株中BmAtlatin-n，BmTHY，Bmseroin1和Bmseroin2的表达相对稳定或上调，进一步表明了它们在体内的抗病毒作用。此外，BmFerHCH，BmNHR96和BmSINAL10的显着下调在BmNPV感染后的两个菌株中，这两个菌株均显示出它们在有益病毒感染以及YeB中肠中BmFerHCH和YeB血淋巴中BmSINAL10上调中的作用。这些数据可以用来补充这些基因对BmNPV感染的功能证明。