The polymer wrapped magnetic nanoparticles (Fe₃O₄) for the controlled release of drugs to the specific targeted site is one of the most effective way. In such a way, we have constructed a Fe₃O₄ magnetic nanoparticle wrapped chitosan (CS) polymer loaded with Ocimum tenuiflorum extract (phyto drug) for the drug delivery applications. The prepared composite nanomaterial is thoroughly characterized for understanding its crystal structure and surface morphology. Further, the zebra fish (Danio rerio) is chosen for the in vivo toxicity investigation of drug loaded Fe₃O₄/CS nanospheres. The phyto drug used has proven to have an excellent activity through our studies on zebra fish. Therefore, our designed drug loaded Fe₃O₄/CS nanospheres can be advocated for the real time diagnosis.

聚合物包裹的磁性纳米粒子（Fe ₃ O ₄）是控制药物释放到特定目标部位的最有效方法之一。通过这种方式，我们构建了Fe ₃ O ₄磁性纳米颗粒包裹的壳聚糖（CS）聚合物，该聚合物负载了Tenciflorum提取物（植物药），用于药物递送应用。对制备的复合纳米材料进行了彻底表征，以了解其晶体结构和表面形态。此外，选择斑马鱼（Danio rerio）进行载药Fe ₃ O _4的体内毒性研究。/ CS纳米球。通过对斑马鱼的研究，已证明所使用的植物药具有出色的活性。因此，我们设计的载药Fe ₃ O ₄ / CS纳米球可用于实时诊断。