Sheep primary epithelial cells are short-lived in cell culture systems. For long-term in vitro studies, primary cells need to be immortalized. This study aims to establish and characterize T immortalized sheep embryo kidney cells (TISEKC). In this study, we used fetal lamb kidneys to derive primary cultures of epithelial cells. We subsequently immortalized these cells using the large T SV40 antigen to generate crude TISEKC and isolate TISEKC clones. Among numerous clones of immortalized cells, the selected TISEKC-5 maintained active division and cell growth over 20 passages but lacked expression of the oncogenic large T SV40 antigen. Morphologically, TISEKC-5 maintained their epithelial aspect similar to the parental primary epithelial cells. However, their growth properties showed quite different patterns. Crude TISEKC, as well as the clones of TISEKC proliferated highly in culture compared to the parental primary cells. In the early passages, immortalized cells showed heterogeneous polyploidy but in the late passages the karyotype of immortalized cells became progressively stable, identical to that of the primary cells, because the TISEKC-5 cell line has lost the large SV40 T antigen expression, this cell line is a valuable tool for veterinary sciences and biotechnological productions.

绵羊原代上皮细胞在细胞培养系统中寿命很短。对于长期体外研究，原代细胞需要永生化。本研究旨在建立和表征 T 永生化绵羊胚胎肾细胞 (TISEKC)。在这项研究中，我们使用胎羊肾脏来获得上皮细胞的原代培养物。我们随后使用大 T SV40 抗原使这些细胞永生化以生成粗 TISEKC 并分离 TISEKC 克隆。在无数永生化细胞克隆中，选定的 TISEKC-5 保持活跃的分裂和细胞生长超过 20 代，但缺乏致癌大 T SV40 抗原的表达。在形态学上，TISEKC-5 保持其与亲本原代上皮细胞相似的上皮外观。然而，它们的生长特性表现出截然不同的模式。原油 TISEKC，以及与亲代原代细胞相比，TISEKC 的克隆在培养中高度增殖。在早期传代中，永生化细胞表现出异质多倍体，但在晚期传代中，永生化细胞的核型逐渐稳定，与原代细胞的核型相同，因为 TISEKC-5 细胞系失去了大的 SV40 T 抗原表达，这种细胞line 是兽医科学和生物技术生产的宝贵工具。