An analytical method based on UHPLC-PDA and LC–ESI–MS/MS was developed for the simultaneous and reliable determination of 22 antidepressant and anti-anxiety pharmaceutical compounds in adulterated functional foods. The method was validated in terms of specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), method detection limit (MDL), method quantitation limit (MQL), precision, accuracy, recovery, stability, and matrix effect. For solid and liquid blank samples, the developed method had LOD = 0.10–1.01 μg/mL for UHPLC and 0.01–5.05 ng/mL for LC–ESI–MS/MS, respectively, LOQ = 0.30–3.02 μg/mL and 0.03–15.15 ng/mL, and R2 > 0.999 and R2 > 0.99. Recoveries from spiked blank samples in powder, liquid, tablet, and capsule forms were determined to be 80.13–113.76%. The precision, accuracy, and stability were acceptable, and the matrix effect was insignificant. A total of 118 real samples were successfully analysed using the proposed rapid screening method.

中文翻译：

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应用同时验证的 UHPLC-PDA 和 LC-ESI-MS/MS 方法测定食品基质样品中的 22 种抗抑郁药和抗焦虑药

开发了一种基于 UHPLC-PDA 和 LC-ESI-MS/MS 的分析方法，用于同时可靠地测定掺假功能食品中的 22 种抗抑郁和抗焦虑药物化合物。该方法在特异性、线性、检测限 (LOD)、定量限 (LOQ)、方法检测限 (MDL)、方法定量限 (MQL)、精密度、准确度、回收率、稳定性和基质效应方面进行了验证. 对于固体和液体空白样品，所开发方法的 UHPLC LOD = 0.10–1.01 μg/mL 和 LC–ESI–MS/MS 的 LOD 分别为 0.01–5.05 ng/mL，LOQ = 0.30–3.02 μg/mL 和 0.03– 15.15 ng/mL，并且 R2 > 0.999 和 R2 > 0.99。粉末、液体、片剂和胶囊形式的加标空白样品的回收率测定为 80.13-113.76%。精密度、准确度和稳定性是可以接受的，且基质效应不显着。使用建议的快速筛选方法成功分析了总共 118 个真实样品。